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Moving while sticking

Contraction and expansion of membranes reorganizes cells as the tissue extends (top to bottom). Anterior is left; posterior is right. Lecuit/Macmillan

Intercalation—the slotting of cells in between one another—is an established method for converting short and fat into long and slim. This is all very well in slippery, adhesion-sparse mesenchymal tissues. But many such extension events occur in epithelial tissues, where cells are glued together by adhesion complexes. Claire Bertet, Lawrence Sulak, and Thomas Lecuit (IBDM, Marseille, France) now report that epithelial intercalation relies on some strategic tugging by myosin that remodels junctions.

The French group expected that extension in fly embryos would involve a few individual motile cells nosing between other stationary cells. Instead they saw "a global and ordered reorganization," says Lecuit. Borders between cells that lay anterior and posterior to each other contracted to a point. Perpendicular expansion of this point generated a border between dorsal and ventral cells, thus pushing the anterior cell more anterior and the posterior cell more posterior. Extension was therefore achieved by a geometrical shuffling of the original hexagonal arrangement.

Myosin II was concentrated near the shrinking (anterior–posterior) membranes and reduced near the expanding (dorsal–ventral) membranes. In embryos with less myosin II, junctions froze and intercalation failed. Myosin need only destabilize adhesion proteins at anterior–posterior membranes, as junction proteins are naturally very dynamic.

It is not known how myosin is concentrated preferentially near anterior and posterior membranes. Genes that define anterior–posterior polarity of the fly embryo are needed; they may exert this effect by either local (cell-to-cell) or global (gradient) messages.

These polarity cues result in an intercalation method that, says Lecuit, maintains "the balance between stability and dynamics." It can account for a nearly twofold extension. If more extension is needed, for a gut tube or an arm, then cell shape changes and oriented cell division may come into play.

Reference:

Bertet, C., et al. 2004. Nature. 429:667–671.[CrossRef][Medline]

William A. Wells

wellsw{at}rockefeller.edu

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This Article Full Text (PDF, 682K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wells, W. A. Search for Related Content PubMed Articles by Wells, W. A. Social Bookmarking                            What's this?