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Patches ride cables

Actin patches (green) around endocytosed material (red) ride actin cables out of the bud (left to right).

On page 519, Huckaba et al. show that endosomes ride an actin-based conveyor belt from bud to mother cell in yeast. This passive mode of transport may replenish membrane fusion components needed for polarized secretion.

Lots of polarized secretion is needed to build a new bud. Secretory vesicles are brought into the bud via active transport along actin cables. These cables are actin bundles that are lengthened by the addition of new actin at the bud site, which thereby produces a rearward flow of actin into the mother cell.

This rearward flow is now shown to carry another actin structure, called an actin patch, into the mother cell. Using an endocytosed label, the authors demonstrate that patches are the actin-rich coats of endosomes. After budding off the membrane at secretion sites, where actin patches are enriched, many endosomes latched onto actin cables and rode them into the mother cell at the rate of the cables' rearward actin flow. Retrograde actin flow is common among higher eukaryotes, where it may also drive vesicle movement.

The patches disassembled when the vesicles met, and presumably fused with, endosomal sorting compartments, from which SNAREs and other secretion proteins can be recycled. Some endosomes avoided cables and remained in the bud. Perhaps the ability to latch onto cables is only present in vesicles containing certain cargoes.

Nicole LeBrasseur

lebrasn{at}rockefeller.edu

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Related Article

Live cell imaging of the assembly, disassembly, and actin cable–dependent movement of endosomes and actin patches in the budding yeast, Saccharomyces cerevisiae

Thomas M. Huckaba, Anna Card Gay, Luiz Fernando Pantalena, Hyeong-Cheol Yang, and Liza A. Pon
J. Cell Biol. 2004 167: 519-530. [Abstract] [Full Text] [PDF]

This Article Full Text (PDF, 800K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by LeBrasseur, N. Search for Related Content PubMed Articles by LeBrasseur, N. Related Collections Related Article Social Bookmarking                            What's this?