Home | Help | Feedback | Subscriptions | Archive | Search | Table of Contents

This Article Full Text (PDF, 419K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tuma, R. S. Search for Related Content PubMed Articles by Tuma, R. S. Social Bookmarking                            What's this?

Dynein holds still

Runt RNA (red) disperses (left to right) after injection of anti-dynein antibody. DAVIS/ELSEVIER

Dynein, well-known for moving cargo down microtubules, is now also shown to anchor its cargo at the target site, according to results from Renald Delanoue and Ilan Davis (University of Edinburgh, UK).

Dynein's cargoes include the wingless and runt mRNAs, which concentrate at the apical tip of fly embryos. Delanoue and Davis found that agents that disrupt microtubule function caused the mRNAs to disperse. Injection of antibodies against dynein subunits disrupted localization of both injected exogenous RNAs and endogenous transcripts, indicating that dynein itself was doing the anchoring.To distinguish between static anchoring and continuous localization, the team used a two-step injection design. They injected one batch of labeled RNA, allowed it to localize, and then injected a second batch along with an ATPase inhibitor to block dynein motility. Under these conditions, the previously localized RNA stayed put, while the newly injected RNA failed to localize. The team thus concluded that dynein remains with its cargo and anchors it at the target site.

"We'd like to think the use of a motor for anchoring is a general mechanism both in terms of other cargoes and other motors," says Davis. "There could be a whole range of transport and anchoring mechanisms involving different motors." He points out that motors are abundant in most cells and could provide a convenient tether after transport.

Reference:

Delanoue, R., and I. Davis. 2005. Cell. 122:97–106.[Medline]

Rabiya S. Tuma

rabiya{at}nasw.org

CiteULike    Complore    Connotea    Del.icio.us    Digg    Facebook    Reddit    Technorati    Twitter    What's this?

This Article Full Text (PDF, 419K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tuma, R. S. Search for Related Content PubMed Articles by Tuma, R. S. Social Bookmarking                            What's this?