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Mixed-up DNA

Two chomosomes (red and green) mingle. POMBO

Interphase human chromosomes show significant intermingling, say Miguel Branco and Ana Pombo (MRC Clinical Sciences Centre, London, UK). The idea is a counter to the most extreme view of chromosome territories.

Territories were so named based on microscopists' early observations: that individual chromosomes did not spread out over an entire nucleus. DNA labeling also indicated that nuclei had gaps between DNA, although it was not clear if these were between DNA of the same or different chromosomes. Still, this was enough to spark talk of an interchromatin domain separating territories. "In this model, it will always be right because two pieces of chromosome will never be in exactly the same place," says Pombo. "The field couldn't move forward."

She and Branco responded by labeling two different chromosomes in ultrathin slices and following up with EM for even higher resolution. Based on extrapolation, an average of 46% of each chromosome territory intermingles with other chromosomes. Furthermore, extents of intermingling for different chromosomes correlated with translocation frequencies.

The nucleus is not a complete tangled mess, however. "Chromosomes as polymers will tend to expand," says Pombo. "But what stops them is interaction with each other and with the nuclear membrane." She is now seeing if transcription factories help keep particular chromosomes fixed to themselves or each other.

Reference:

Branco, M.R., and A. Pombo. 2006. PLoS. 4:e138.

William A. Wells

wellsw{at}rockefeller.edu

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This Article Full Text (PDF, 740K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wells, W. A. Search for Related Content PubMed Articles by Wells, W. A. Social Bookmarking                            What's this?