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Virus squeezed shut

In a full virion, a ring of DNA (green) squeezes the gp1 portal (red) into a new conformation. JOHNSON/AAAS

The DNA in a fully packed viral capsid squeezes a pressure sensor, as revealed by images from Gabriel Lander, John Johnson (Scripps Research Institute, La Jolla, CA), and colleagues. The embrace triggers the end of DNA packaging.

Each particle of the p22 bacteriophage, a herpes cousin, contains a single copy of its genome. The DNA is pumped as a long concatamer into one end of the capsid through a portal of gp1 proteins. After one genome enters, the concatamer is cleaved and the portal plugged shut. In gp1 mutants, too much DNA is let in, but just how the wild-type portal senses full capacity was unknown.

Johnson's group viewed the fully assembled portal using automated electron microscopy, which identified items of interest systematically, thus retrieving ten times as many images as in manual reconstructions. The higher resolution data revealed features of the intact viral particle that were previously hidden, including the gp1 portal. "Everything we dreamed of seeing was staring us in the face," said Johnson.

The portal had previously been seen as an isolated entity, but it looked much different in the DNA-filled virion. A ring of DNA wrapped around the portal "sort of like a belt," said Johnson. "It looks like the DNA is squeezing the portal and changing its conformation. This probably signals to the outside, ‘we are full of DNA.’" The pumps then know to cut the DNA.

Reference:

Lander, G.C., et al. 2006. Science. doi:10.1126/science.1127981.[Abstract/Free Full Text]

Nicole LeBrasseur

lebrasn{at}rockefeller.edu

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This Article Full Text (PDF, 883K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by LeBrasseur, N. Search for Related Content PubMed Articles by LeBrasseur, N. Social Bookmarking                      What's this?