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T cells (green) interact (left) with antigen-bearing DCs (red). T cells spend little time with DCs (filled triangles, right) that carry little antigen but linger longer (open triangles) when high antigen–density DCs are also present. VON ANDRIAN/HARVARD

Tcells tabulate the density of antigens in their surroundings before deciding whether to activate, say Sarah Henrickson, Ulrich von Andrian (Harvard Medical School, Boston, MA), and colleagues.

In a prior study, von Andrian says, his group had noticed T cells within lymph nodes flit "like bumblebees" from one antigen-presenting dendritic cell (DC) to another. Now they have found that when antigens are scarce, it takes many hours for them to settle down and connect with DCs, the prelude to full activation.

To determine the rules governing this transition to a stationary phase, the authors injected foreign T cells and observed them in a lymph node directly behind the knee in mice. When they also injected foreign DCs carrying labeled antigen into the footpad, they could watch as T cells and DCs met in the lymph node.

They found that the more antigen in the environment, the less time a T cell spent flitting around before latching onto a DC. Using DCs with differing antigen density, they showed that T cells would rapidly settle down with low-antigen density DCs, as long as they had previously encountered high amounts of antigen before.

"It was the total dose exposure, not the dose on any one dendritic cell, that triggered the response," von Andrian says. "The T cell has to make a binary decision, but antigen dose is an analogue signal, dependent on number and density. Somehow, the T cell is able to integrate that signal from its multiple encounters." This feature would allow the T cell to respond when there is a lot of low-affinity antigen, as might occur during an infection. "A T cell can make an informed decision only by remembering how much antigen it has been exposed to. Now we must understand how it does so."

Reference:

Henrickson, S.E., et al. 2008. Nat. Immunol. doi:10.1038/ni1559.[Abstract/Free Full Text]

Richard Robinson

rrobinson{at}nasw.org

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This Article PDF (Full Text) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Robinson, R. Search for Related Content PubMed Articles by Robinson, R. Social Bookmarking                      What's this?