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Article |
TLR ligand–induced podosome disassembly in dendritic cells is ADAM17 dependent
Correspondence to Colin Watts: c.watts{at}dundee.ac.uk
Toll-like receptor (TLR) signaling induces a rapid reorganization of the actin cytoskeleton in cultured mouse dendritic cells (DC), leading to enhanced antigen endocytosis and a concomitant loss of filamentous actin–rich podosomes. We show that as podosomes are lost, TLR signaling induces prominent focal contacts and a transient reduction in DC migratory capacity in vitro. We further show that podosomes in mouse DC are foci of pronounced gelatinase activity, dependent on the enzyme membrane type I matrix metalloprotease (MT1-MMP), and that DC transiently lose the ability to degrade the extracellular matrix after TLR signaling. Surprisingly, MMP inhibitors block TLR signaling–induced podosome disassembly, although stimulated endocytosis is unaffected, which demonstrates that the two phenomena are not obligatorily coupled. Podosome disassembly caused by TLR signaling occurs normally in DC lacking MT1-MMP, and instead requires the tumor necrosis factor
–converting enzyme ADAM17 (a disintegrin and metalloprotease 17), which demonstrates a novel role for this "sheddase" in regulating an actin-based structure.
, tumor necrosis factor ; WASp, Wiskott-Aldrich syndrome protein; WIP, WASp-interacting protein. © 2008 West et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
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Introduction |
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These changes can be viewed as adaptations that boost the performance of DC as antigen-capturing, -processing, and -presenting cells. For example, transiently enhanced antigen capture at the time and place of microbial product sensing should increase the display of pathogen-derived peptides to T cells. Consistent with this, simultaneous exposure of DC to antigen and TLR ligand led to enhanced T cell stimulation compared with sequential exposure to first antigen and then TLR ligand (West et al., 2004). The possible significance of other rapid morphological changes triggered by TLR signaling is less obvious, however. In particular, we observed that TLR ligand–enhanced macropinocytosis was accompanied by a striking loss of F-actin–rich podosomes. As the rate of macropinocytosis subsequently fell, podosomes were reassembled, which suggested a reciprocal relationship and possibly a redeployment of cytoskeletal resources from podosomes to actin-rich ruffles and macropinosomes (West et al., 2004). Podosomes were also lost when human DC were stimulated with the TLR4 ligand lipopolysaccharide (LPS), but on a longer time scale compared with mouse DC (Burns et al., 2004; van Helden et al., 2006).
The biological significance of TLR ligand–induced podosome disassembly and, indeed, the biological functions of podosomes generally, are still uncertain. Podosomes have a striking morphology and are found in cells derived from the monocyte lineage, including osteoclasts, macrophages, and DC, as well as in endothelial cells and Src- and Rsv-transformed fibroblasts (Linder and Aepfelbacher, 2003; Buccione et al., 2004; Linder, 2007; Saltel et al., 2008). Podosomes are related to invadopodia found in invasive tumor cells, although the cellular and extracellular factors that induce one as opposed to the other structure are unclear. Podosomes consist of a core of F-actin and actin-regulatory proteins typically surrounded by a ring or matrix of other proteins, including integrins, paxillin, vinculin, and talin (Linder and Aepfelbacher, 2003). Many other proteins have been localized to podosomes, including, at least in osteoclasts and endothelial cells, matrix metalloproteases (MMPs). Several different MMPs have been reported to localize to podosomes and to invadopodia and, particularly in the latter, are responsible for localized degradation of the adjacent matrix (Linder, 2007). The F-actin core of podosomes turns over rapidly (t1/2 = 
30 s), and individual podosomes have short lifetimes of a few minutes (Destaing et al., 2003; Evans et al., 2003). Podosomes and invadopodia have been suggested to be involved in cell migration and invasion (Linder and Aepfelbacher, 2003; Buccione et al., 2004; Linder, 2007). For this reason, the presence of podosomes in DC and their acute regulation by TLR signaling is of considerable interest.
Here, we investigate the significance and mechanism of reversible TLR ligand–induced disassembly of podosomes in mouse DC. Our results are consistent with the idea that DC migration and antigen sampling may be mutually exclusive activities, and they support the proposal that podosomes and specific podosome-localized MMP activities contribute to DC emigration from tissues. Membrane type I MMP (MT1-MMP) and, unexpectedly, a disintegrin and metalloprotease 17 (ADAM17) are involved in distinct aspects of DC podosome function and regulation.
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90 min, we kept the time allowed for migration as short as possible. We found that 5–10% of DC migrated through a Transwell chamber in 2 h. We avoided the use of chemokine attractants because the expression of chemokine receptors changes after TLR signaling (Sallusto and Lanzavecchia, 2000). To internally control the experiment, we included DC from both C3H/HeN and C3H/HeJ mice. The latter do not respond acutely to LPS because of a point mutation in TLR4 (Poltorak et al., 1998). Consequently, C3H/HeJ DC show no stimulation of pinocytosis, nor do they show any podosome loss in response to LPS (Fig. S1, available at http://www.jcb.org/cgi/content/full/jcb.200801022/DC1). We labeled BMDC from C3H/HeJ and from LPS-responsive C3H/HeN mice with different fluorescent dyes, mixed them in a 1:1 ratio, and placed them in the top half of Transwell chambers. The cells were exposed to LPS immediately before their transfer to the Transwell. After a 2 h period, cells that had migrated to the lower chamber were recovered (see Materials and materials), quantitated by flow cytometry, and expressed as a ratio (HeN/HeJ). As shown in Fig. 2 A, almost threefold fewer LPS-sensitive HeN cells migrated compared with HeJ cells, which demonstrates that TLR signaling compromised the ability of the cells to migrate.
We repeated the experiment over a longer time course of LPS treatment. As before, when migration was measured immediately after LPS challenge, substantially fewer HeN cells had migrated compared with HeJ cells (decreased HeN/HeJ ratio; Fig. 2, B and C). However, after longer LPS exposure, the HeN cells resumed their migratory activity, and the migration ratio returned to and in some experiments exceeded 1 (Fig. 2, B and C). Collectively, these experiments show that TLR-stimulated DC experience a transient loss of migratory capacity in vitro, which coincides with the phase of acute stimulation of antigen capture, cell spreading, and podosome loss.
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MT1-MMP is responsible for the in vitro matrix-degrading activity of DC podosomes
To identify the GM6001-sensitive activity responsible for podosome-associated matrix degradation, we investigated which MMPs are expressed in cultured mouse DC and tested DC from mice lacking selected MMPs. MMPs 8, 9, 12, 13, 14, and 19 were all expressed in BMDC as determined by microarray experiments (unpublished data). In addition, quantitative PCR data indicated that cultured SDC additionally expressed MMPs 2, 3, and 28 (unpublished data). The presence of TGFβ during cell culture may account for the additional MMPs expressed in SDC. MMP2 or MMP9 are gelatinases, but neither were absolutely required because DC from mice lacking one or other of these MMPs showed no obvious defect in matrix degradation (Fig. 4 A).
We also tested DC from mice lacking membrane-anchored MT1-MMP (MMP14) because this activity has been associated with invadopodia in invasive tumor cells (Nakahara et al., 1997). MT1-MMP–deficient mice are severely affected by the loss of this key MMP activity. They are not born at the expected Mendelian ratio, have multiple skeletal and connective tissue abnormalities, and die 2 wk after birth (Holmbeck et al., 1999; Zhou et al., 2000). We set up DC cultures from 10–14-d-old MT1-MMP–deficient mice alongside DC from wild-type littermates. The growth of DC in culture did not seem to be affected by the loss of MT1-MMP. However, compared with wild-type DC, gelatin matrix–degrading activity in the MT1-MMP–null DC was almost completely lost (Fig. 4 B). This striking result was observed in both BMDC and in SDC. Quantitation of the degradation of the underlying matrix confirmed that MT1-MMP–deficient DC are profoundly compromised in their ability to degrade a gelatin matrix, even over an 8-h time course (Fig. 4 C), although normal levels of podosomes were observed (Fig. 4 D).
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(TNF-) from the DC surface (unpublished data). This finding allowed us to test the hypothesis that TLR ligand–stimulated macropinocytosis might be dependent on podosome disassembly, e.g., to release additional actin resources. Cultured mouse DC were stimulated with Pam3CSK in the presence or absence of TAPI-1, and macropinocytosis was measured by FITC-dextran uptake (West et al., 2004). In fact, blockade of podosome disassembly with the MMP inhibitor had little effect on LPS-stimulated endocytosis (Fig. 5 C). Furthermore, the presence of an MMP inhibitor had little effect on the TLR ligand–induced increase in prominent focal contacts (Fig. 5, D and E) or ventral cell surface area (not depicted). These results lead to two important conclusions. First, neither the stimulated endocytic response nor the increase in focal contacts and cell spreading is obligatorily coupled to the disassembly of podosomes. Second, MMP activity is not only organized at podosomes for purposes of matrix degradation but can additionally initiate podosome loss after TLR signaling.
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PGE2 has been reported to rapidly induce podosome disassembly in human DC (van Helden et al., 2006), a finding we confirmed in mouse DC (Fig. 5 G). However, this response was insensitive to MMP inhibitors, demonstrating that TLR ligands and PGE2 destabilize podosomes by distinct mechanisms. PMA also triggered podosome disassembly in DC and, similar to TLR-driven disassembly, this was also dependent on MMP activity (Fig. 5 G).
TLR-induced podosome disassembly requires a distinct MMP activity
We asked which MMP activities are required for TLR ligand–induced podosome disassembly. Interestingly, LPS induced loss of podosomes to the same extent in wild-type and in MT1-MMP–deficient DC, which indicates that matrix degradation and podosome stability are controlled by different MMP activities (Fig. 6 A).
Furthermore, MMP2 and MMP9 were also ruled out because DC lacking these enzymes disassembled podosomes normally in response to LPS (Fig. 6 B). MMP activities might destabilize podosomes by proteolytic cleavage or "shedding" of key proteins, e.g., integrins, that mediate substrate attachment at podosomes. Such activities could be recruited and/or activated after TLR signaling. To further define the MMP activities responsible and test whether known sheddases such as ADAM17 (also known as TNF-–converting enzyme [TACE]) and ADAM10 might be involved, we tested two additional selective inhibitors of these enzymes: GW280264X (GW), which blocks both ADAM10 and ADAM17; and GI254023X (GI), which inhibits ADAM10 100-fold more effectively than ADAM17 (Hundhausen et al., 2003; Chalaris et al., 2007). TLR signaling is known to stimulate TNF- release from its membrane-bound precursor form in an ADAM17-dependent manner (Black et al., 1997), providing a convenient assay for these inhibitors. As before, DC were stimulated with a TLR ligand (Pam3CSK) in the presence or absence of these inhibitors, and both TNF- release and podosome loss were monitored. TNF- release from the cells was potently inhibited by the GW compound but only slightly by GI, which is consistent with the known involvement of ADAM17 but not ADAM10 in stimulated TNF- release (Fig. 6 C). Podosome loss closely mirrored this response, being blocked by GW but not by GI (Fig. 6 D), which is consistent with a possible involvement of ADAM17.
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was also substantially reduced in DC from the ADAM17fl/fl mice (Fig. 7 B), although the actual deficit was variable among DC cultures generated from different mice. Importantly, DC from the ADAM17fl/fl mice showed a striking inability to disassemble podosomes in response to TLR signaling (Fig. 7, C and D). In DC from different mice, this ranged from virtually complete inhibition to 50% inhibition of the response. When we compared the percentage of inhibition of TLR-induced TNF- release versus the percentage of inhibition of TLR-induced podosome loss in BMDC and SDC from five different ADAM17fl/fl mice, there was a positive correlation between the two (Fig. 7 E; r = 0.76). To confirm that ADAM17 activity was required for podosome disassembly, we attempted to rescue the response by reintroduction of ADAM17 into primary DC from the ADAM17fl/fl mice. As shown in Fig. 7 F, retroviral reexpression of ADAM17 restored protein levels in ADAM17fl/fl mouse DC. Importantly, when DC infected with the ADAM17 virus (GFP+) were stimulated with LPS, the podosome disassembly response was largely restored (Figs. 7, G and H). In contrast, neighboring uninfected (GFP–) cells retained their podosomes (Fig. 7 H). These data demonstrate, unexpectedly, that the TNF- and L-selectin shedding enzyme ADAM17/TACE plays a key role in TLR-induced podosome regulation in mouse DC.
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Our data support the idea that DC transiently lose migratory capacity during acute antigen sampling. Migration through a transwell barrier was impeded after LPS challenge, as shown by the fact that when mixed populations of LPS responsive and nonresponsive DC were monitored, the responsive DC lagged behind. This lag phase coincided with a transient switch in the type of adhesive structure expressed by DC from predominantly podosomes to focal contacts. We suggest that the loss of migratory capacity is due, at least in part, to the loss of actin-rich podosomes and the development of more prominent focal contacts and cell spreading. The role of podosomes in cell migration on planar substrates as opposed to cell invasiveness is still debated. The presence of podosomes at the leading edge of macrophages (Evans et al., 2003) and DC (Burns et al., 2004; Svensson et al., 2008) suggests that they contribute to directed migration, but it is clear that podosomes are not essential for migration on 2D substrates. For example, human DC matured with LPS for 16 h lost podosomes, but their capacity to migrate on 2D surfaces was actually increased (van Helden et al., 2006), and macrophages deficient in Rac2 lacked podosomes but still migrated on a coated glass substrate (Wheeler et al., 2006). Nonetheless, considerable evidence implicates podosomes and invadopodia in cell migration in three dimensions, e.g., through an extracellular matrix barrier. MMP activity organized by podosomes and invadopodia is thought to be a key element in this process (Linder, 2007). Although no defined matrix barrier was used in our short-term migration experiments, podosomes may help the cell to negotiate the filter barrier. This may be because MMPs have additional roles besides pericellular matrix proteolysis that may also aid migration (Mott and Werb, 2004; Van Lint and Libert, 2007), e.g., cytokine and chemokine processing and shedding of cell surface proteins such as syndecan-1 (Endo et al., 2003) and CD44 (Nakamura et al., 2004). These additional MMP functions may be most effective when focused at the advancing cell boundary. We recently found that disruption of normal ARF6 GTPase activity strongly inhibited both podosome formation and immature DC migration in vitro, again linking these structures to DC migratory capacity (Svensson et al., 2008).
How might the TLR ligand–induced loss and subsequent regain of podosomes be rationalized in the context of DC behavior in vivo? Under noninfectious resting conditions, DC are known to exit tissues at a basal rate and travel to lymphoid organs (Huang et al., 2000; Lanzavecchia and Sallusto, 2001). These constitutively migrating DC are believed to be important in the induction of tolerance to self-antigens (Heath and Carbone, 2001; Steinman et al., 2003). We propose that DC use podosomes or related structures as part of the machinery needed for DC mobilization and exit from tissues under these conditions (Fig. 8). Upon detection of an infectious or inflammatory stimulus, DC would initiate a transient phase of enhanced actin-dependent capture of local antigens that, we propose, may be facilitated by loss of migratory capacity as a result of a switch in the dominant adhesive structure, i.e., podosomes to focal contacts. At the end of this period of acutely stimulated endocytosis, podosomes may reform, and migration of antigen-loaded DC could resume (Fig. 8). The microbial and inflammatory stimuli encountered also serve to switch the DC from a tolerogenic to an immunogenic mode (Lanzavecchia and Sallusto, 2001; Reis e Sousa, 2006). Though obviously speculative, the model implies that DC undergo a transient loss of migratory capacity during the phase of acute antigen endocytosis, a prediction that is at least consistent with the in vitro experiments described here.
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We identified an unexpected and novel relationship between MMP activity and podosome genesis and stability. The presence of MMP inhibitors completely blocked TLR ligand–induced loss of podosomes. Interestingly, the relevant MMP activity was not MT1-MMP, as podosomes disassembled normally in LPS-treated DC from MT1-MMP–deficient mice, but rather was the TNF-–converting enzyme ADAM17. To our knowledge this is the first time that this activity has been implicated in the dynamics of actin-based structures associated with cell adhesion and migration. The ADAMs family of proteinases contains the major cell surface sheddases known to cleave cytokines, growth factors, and their receptors, as well as adhesion molecules (Seals and Courtneidge, 2003). Moreover, shedding events such as the release of TNF- by ADAM17 is known to be activated by TLR ligands in a MAP kinase–dependent fashion (Schlondorff and Blobel, 1999; Soond et al., 2005; Rousseau et al., 2008), which is consistent with our finding that TLR-triggered podosome disassembly is blocked by MAP kinase inhibitors (West et al., 2004).
The precise mechanistic link between ADAM17 and TLR ligand–triggered podosome disassembly remains to be elucidated. Most likely, TLR signaling induces the recruitment to the cell surface and/or activation of ADAM17, which then cleaves key surface proteins required for cell–substrate contact and podosome formation. The identity of these proteins remains to be established. Podosome reappearance may require the repopulation of the cell surface with those proteins. Recently, the intracellular protease calpain has also been implicated in podosome stability. Calpain inhibitors increased recruitment of integrins and Wiskott-Aldrich syndrome protein (WASp) to podosomes, apparently by blocking calpain-mediated cleavage of WASp (Calle et al., 2006). A follow-up study (Chou et al., 2006) showed that DC lacking WASp-interacting protein (WIP) also lack normal podosomes, as do WASp-deficient DC (Burns et al., 2004). WIP appears to regulate both the location and stability of WASp and is localized in podosome actin cores (Chou et al., 2006). Interestingly, WIP–/– DC had more pronounced focal contacts and membrane ruffles compared with wild-type DC, which is similar to the state transiently induced by TLR signaling. However, we found that calpain inhibitors did not affect the sensitivity of DC podosomes to TLR signaling (unpublished data), which indicates that perturbation of the calpain–WASp–WIP system is not likely to be the primary target of LPS signaling. Apparently, both the calpain and MMP systems can influence podosome stability.
Having found a way to selectively inhibit part of the TLR ligand–induced cytoskeleton response, we were able to test the possibility that podosome disassembly might be necessary to fuel increased actin-dependent endocytosis. In fact, macropinocytosis was boosted to almost the same extent in the presence of MMP inhibitors, which demonstrates that the two phenomena are coincident but not obligatorily linked. DC evidently have sufficient actin cytoskeletal resources to maintain significant numbers of podosomes and to develop the pronounced ruffles that give rise to macropinosomes.
In summary, in spite of the increasing attention that podosomes have received, there are few instances where their assembly and disassembly can be triggered over a short time scale and the consequences for the cell assessed. Our data support the idea that podosomes enhance DC migration through three-dimensional space and that their TLR ligand-induced loss might aid local antigen capture by transiently arresting migration. Moreover, we show that MT1-MMP activity is organized by DC podosomes for pericellular proteolysis but that a distinct MMP activity, ADAM17, is involved in their TLR ligand–triggered disassembly. Taken as a whole, these data reveal new features of podosomes and suggest they play an important role in the early DC response to innate immune stimuli.
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Materials and methods |
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10% of ADAM17 expression and activity as compared with wild-type mice. Using a novel strategy, ADAM17fl/fl mice were generated by introducing into the germ line a new exon between exons 11 and 12 so that the new exon started with a translational stop codon. Because we used splice donor/acceptor sites that deviated slightly from the canonical splice donor/acceptor sequence, the new exon was only used in 90–95% of the transcripts, leaving 5–10% of the ADAM17 mRNAs with the wild-type sequence. These 5–10% were enough for the mice to survive. Consequently, ADAM17fl/fl mice show a 90–95% reduction in ADAM17 expression.
Inhibitors, TLR ligands, and stimuli
MMP inhibitors TAPI-1 (BIOMOL International, L.P.), TAPI-2 (BIOMOL International, L.P.), GM6001 (EMD), and CT1746 (G. Murphy, University of Cambridge, Cambridge, England, UK) were all used at 25 µM. GW280264X and GI254023X were both used at 3 µM. Stimulation was performed with TLR ligands LPS (50 ng/ml; Qbiogene) and Pam3CSK (100 ng/ml; EMC Microcollections), or with PMA (30 ng/ml; Sigma-Aldrich) or PGE2 (10 µg/ml; Qbiogene).
Light microscopy
For visualization and quantitation of cell adhesions, 2 x 105 DC that had been plated onto glass coverslips were fixed with 4% paraformaldehyde in PBS, permeabilized with 0.2% Triton X-100, and costained with TRITC-phalloidin (Sigma-Aldrich) for F-actin and anti-vinculin (hVin-1; Sigma-Aldrich), followed by Alexa 488– or 633–goat anti–mouse IgG (Invitrogen). Single optical sections of 0.7 µm, taken at the ventral surface of the cells, were acquired on a confocal microscope (LSM 510 META) using a 100x PlanFluar/NA 1.45 objective and LSM 510 software (all from Carl Zeiss, Inc.). Time-lapse video microscopy of BMDC expressing GFP-actin was performed using a 37°C temperature-controlled chamber flushed with 5% CO2 in air, and images were collected using the microscope and objective as before. For matrix degradation, z stacks of 10 x 0.7-µm sections were acquired every 2 min, taken at 0.34-µm intervals. For each time point, the stacks were converted into a single projection using software (Carl Zeiss, Inc.), and the time series was converted into a QuickTime video (Apple). For analysis of podosome lifetimes, 5 x 0.7-µm sections at 0.34-µm intervals were acquired every 20 s. Maximum projections for each z series, for 50 sequential frames, were imported into the Imaris software (Bitplane AG) for analysis as described in the following section.
Quantitation
Quantitation of the percentage of cells with podosomes or with focal contacts was performed using cells stained with TRITC-phalloidin and anti-vinculin. 200–300 cells per condition per experiment were assessed for podosomes and 150–200 for focal contacts.
For quantitation of the number of focal contacts or of the ventral cell surface area, images collected using identical acquisition parameters were analyzed using Volocity software (PerkinElmer). Focal contacts were selected by intensity of green fluorescence (vinculin) and distinguished from podosomes based on size. The total number of focal contacts for individual complete cells (defined by regions of interest) in each image was then calculated. For measurement of the ventral surface areas, all of the cells in an image were first identified by fluorescence in the red channel (TRITC-phalloidin staining of cortical actin). This selected the precise area of the cells. To extract this data for individual cells in the field, each complete cell was selected using a simple region of interest. The mean area per cell for each condition was then calculated.
For measurement of podosome lifetimes, time sequences were analyzed using Imaris software by a modification of the methods of Evans et al. (2003). In brief, the time sequences were converted to kymographs by swapping time for z and filtered to remove background fluorescence and noise. The images were then cut into uniformly sampled yz slices of 10 pixels, with 5-pixel intervals between the slices, starting at a random point (see Fig. S3). All podosome lives in the kymographs slices were measured regardless of whether they originated outside the time window or continued beyond this window. The total lifetime for all podosomes scored in these cell slices was then divided by the total number of podosome initiation events to give the mean podosome lifetime for each cell.
Transwell migration
BMDC from C3H/HeN or C3H/HeJ mice were labeled with 0.2 µM carboxyfluorescein succinimidyl ester (CFSE) or 2 µM CMTMR (Invitrogen) for 15 min at 37°C, washed three times, and then reincubated for a further 15 min at 37°C. The two populations of cells were mixed in a 1:1 ratio, and 105 mixed cells were placed in the upper chamber of a Transwell 24-well insert (5-µm pore filter; Costar) or directly into the well without an insert (input control). After 2 h at 37°C, the cells that had migrated through the filter were recovered using 10 mM EDTA and stained with an APC-labeled anti-CD11c antibody (HL3; BD Biosciences). Then, the ratio of CFSE- to CMTMR-labeled CD11c+ cells was assessed by flow cytometry using a FACS Calibur (BD Biosciences). Where indicated, DC were pretreated with 50 ng/ml LPS before migration. To normalize the data across a time course, the ratio of migrated HeN/HeJ cells was divided by the input ratio for each time point.
Matrix degradation
Glass coverslips were coated with matrix proteins: 10 µg/ml FITC-gelatin (Invitrogen), 10 µg/ml Alexa 594–gelatin, 50 µg/ml Oregon green–collagen type IV (Invitrogen), or 50 µg/ml Alexa 488–fibronectin and cross-linked with 0.5% glutaraldehyde where indicated (Bowden et al., 2001). Labeling of gelatin (Sigma-Aldrich) and fibronectin (EMD) with Alexa 594 and 488 dyes was performed using the respective protein labeling kits (Invitrogen) according to the manufacturer's instructions. 2 x 105 DC were plated onto matrix-coated coverslips and incubated at 37°C to allow degradation, then fixed with 4% paraformaldehyde in PBS and stained with TRITC-phalloidin. Matrix degradation was quantitated by counting degradation patches in 15 fields of cells (300–500 cells) per condition for each experiment. For time-lapse analysis of matrix degradation, BMDC infected with retrovirus expressing GFP-actin were plated into glass-bottomed dishes (WillCo; Intracel) coated with cross-linked Alexa 594–gelatin.
FITC-dextran uptake
2 x 105 SDC that had been allowed to adhere to glass coverslips for 1–2 h were pretreated with 25 µM TAPI-1, then stimulated with 100 ng/ml Pam3CSK for 30 min before incubation with 1 mg/ml FITC-dextran (Invitrogen) for 10 min at 37°C. Cells were detached with PBS containing 5 mM EDTA, and pinocytosed dextran was measured by flow cytometry (West et al., 2004).
Retroviral constructs and BMDC retroviral infection
Mouse ADAM17 cDNA (Thermo Fisher Scientific) was cloned into the BamHI and XhoI sites of the Moloney murine leukemia virus–based vector pBMN-IRES-GFP (provided by G. Nolan, Stanford University, Stanford, CA). LZRS-pBMN EGFP-actin has been described previously (West et al., 2004). Virus was produced by transfecting the Phoenix Eco 293T packaging cell line with these vectors, and BMDC were infected using viral supernatants as described previously (West et al., 2004).
TNF- shedding
105 DC were plated into 96-well plates in 100 µl of complete RPMI and stimulated with 50 ng/ml LPS or 100 ng/ml Pam3CSK for 2 h, then 10 µl of the medium was assayed for secreted TNF- by ELISA (Peprotech), according to the manufacturer's instructions.
Cell lysis and immunoblot
DC were lysed in SDS sample buffer. Equal amounts of proteins were separated by electrophoresis on 4–12% NuPage gels (Invitrogen), then transferred onto nitrocellulose membrane (GE Healthcare) and probed with antibodies against ADAM17 (Spring Bioscience) and actin (AC-40; Sigma-Aldrich).
Statistical analysis
Error bars represent SEM unless otherwise stated. The unpaired two-tailed t test was used for statistical analysis.
Online supplemental material
Fig. S1 shows that DC from C3H/HeJ mice are insensitive to LPS stimulation of FITC-dextran pinocytosis or podosome disassembly. Fig. S2 shows podosome-associated degradation of gelatin, collagen type IV, and fibronectin. Fig. S3 illustrates the method used for measurement of podosome lifetimes. Video 1 shows spreading, ruffling, and pinocytosis in DC in response to LPS treatment. Video 2 shows degradation of gelatin matrix by DC expressing GFP-actin. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200801022/DC1.
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Acknowledgments |
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We are grateful to the Medical Research Council for program grant GO200536 (to C. Watts and A.R. Prescott) and the Hong Kong Research Grant Council (grant CERG 751303 to Z. Zhou) for funding.
Submitted: 7 January 2008
Accepted: 4 August 2008
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References |
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TopAbstractIntroductionResultsDiscussionMaterials and methodsReferences |
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Black, R.A., C.T. Rauch, C.J. Kozlosky, J.J. Peschon, J.L. Slack, M.F. Wolfson, B.J. Castner, K.L. Stocking, P. Reddy, S. Srinivasan, et al. 1997. A metalloproteinase disintegrin that releases tumour-necrosis factor-alpha from cells. Nature. 385:729–733.[CrossRef][Medline]
Blander, J.M., and R. Medzhitov. 2004. Regulation of phagosome maturation by signals from toll-like receptors. Science. 304:1014–1018.
Blander, J.M., and R. Medzhitov. 2006. Toll-dependent selection of microbial antigens for presentation by dendritic cells. Nature. 440:808–812.[CrossRef][Medline]
Boes, M., N. Bertho, J. Cerny, M. Op den Brouw, T. Kirchhausen, and H. Ploegh. 2003. T cells induce extended class II MHC compartments in dendritic cells in a toll-like receptor-dependent manner. J. Immunol. 171:4081–4088.
Bowden, E.T., P.J. Coopman, and S.C. Mueller. 2001. Invadopodia: unique methods for measurement of extracellular matrix degradation in vitro. Methods Cell Biol. 63:613–627.[Medline]
Buccione, R., J.D. Orth, and M.A. McNiven. 2004. Foot and mouth: podosomes, invadopodia and circular dorsal ruffles. Nat. Rev. Mol. Cell Biol. 5:647–657.[CrossRef][Medline]
Burns, S., A.J. Thrasher, M.P. Blundell, L. Machesky, and G.E. Jones. 2001. Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation. Blood. 98:1142–1149.
Burns, S., S.J. Hardy, J. Buddle, K.L. Yong, G.E. Jones, and A.J. Thrasher. 2004. Maturation of DC is associated with changes in motile characteristics and adherence. Cell Motil. Cytoskeleton. 57:118–132.[CrossRef][Medline]
Calle, Y., N.O. Carragher, A.J. Thrasher, and G.E. Jones. 2006. Inhibition of calpain stabilises podosomes and impairs dendritic cell motility. J. Cell Sci. 119:2375–2385.
Chalaris, A., B. Rabe, K. Paliga, H. Lange, T. Laskay, C.A. Fielding, S.A. Jones, S. Rose-John, and J. Scheller. 2007. Apoptosis is a natural stimulus of IL6R shedding and contributes to the proinflammatory trans-signaling function of neutrophils. Blood. 110:1748–1755.
Chou, H.C., I.M. Anton, M.R. Holt, C. Curcio, S. Lanzardo, A. Worth, S. Burns, A.J. Thrasher, G.E. Jones, and Y. Calle. 2006. WIP regulates the stability and localization of WASP to podosomes in migrating dendritic cells. Curr. Biol. 16:2337–2344.[CrossRef][Medline]
Chow, A., D. Toomre, W. Garrett, and I. Mellman. 2002. Dendritic cell maturation triggers retrograde MHC class II transport from lysosomes to the plasma membrane. Nature. 418:988–994.[CrossRef][Medline]
Destaing, O., F. Saltel, J.C. Geminard, P. Jurdic, and F. Bard. 2003. Podosomes display actin turnover and dynamic self-organization in osteoclasts expressing actin-green fluorescent protein. Mol. Biol. Cell. 14:407–416.
Endo, K., T. Takino, H. Miyamori, H. Kinsen, T. Yoshizaki, M. Furukawa, and H. Sato. 2003. Cleavage of syndecan-1 by membrane type matrix metalloproteinase-1 stimulates cell migration. J. Biol. Chem. 278:40764–40770.
Evans, J.G., I. Correia, O. Krasavina, N. Watson, and P. Matsudaira. 2003. Macrophage podosomes assemble at the leading lamella by growth and fragmentation. J. Cell Biol. 161:697–705.
Granucci, F., E. Ferrero, M. Foti, D. Aggujaro, K. Vettoretto, and P. Ricciardi-Castagnoli. 1999. Early events in dendritic cell maturation induced by LPS. Microbes Infect. 1:1079–1084.[CrossRef][Medline]
Guermonprez, P., J. Valladeau, L. Zitvogel, C. Thery, and S. Amigorena. 2002. Antigen presentation and T cell stimulation by dendritic cells. Annu. Rev. Immunol. 20:621–667.[CrossRef][Medline]
Heath, W.R., and F.R. Carbone. 2001. Cross-presentation, dendritic cells, tolerance and immunity. Annu. Rev. Immunol. 19:47–64.[CrossRef][Medline]
Holmbeck, K., P. Bianco, J. Caterina, S. Yamada, M. Kromer, S.A. Kuznetsov, M. Mankani, P.G. Robey, A.R. Poole, I. Pidoux, et al. 1999. MT1-MMP-deficient mice develop dwarfism, osteopenia, arthritis, and connective tissue disease due to inadequate collagen turnover. Cell. 99:81–92.[CrossRef][Medline]
Huang, F.P., N. Platt, M. Wykes, J.R. Major, T.J. Powell, C.D. Jenkins, and G.G. MacPherson. 2000. A discrete subpopulation of dendritic cells transports apoptotic intestinal epithelial cells to T cell areas of mesenteric lymph nodes. J. Exp. Med. 191:435–444.
Hundhausen, C., D. Misztela, T.A. Berkhout, N. Broadway, P. Saftig, K. Reiss, D. Hartmann, F. Fahrenholz, R. Postina, V. Matthews, et al. 2003. The disintegrin-like metalloproteinase ADAM10 is involved in constitutive cleavage of CX3CL1 (fractalkine) and regulates CX3CL1-mediated cell-cell adhesion. Blood. 102:1186–1195.
Itoh, T., T. Ikeda, H. Gomi, S. Nakao, T. Suzuki, and S. Itohara. 1997. Unaltered secretion of beta-amyloid precursor protein in gelatinase A (matrix metalloproteinase 2)-deficient mice. J. Biol. Chem. 272:22389–22392.
Kleijmeer, M., G. Ramm, D. Schuurhuis, J. Griffith, M. Rescigno, P. Ricciardi-Castagnoli, A.Y. Rudensky, F. Ossendorp, C.J. Melief, W. Stoorvogel, and H.J. Geuze. 2001. Reorganization of multivesicular bodies regulates MHC class II antigen presentation by dendritic cells. J. Cell Biol. 155:53–63.
Lanzavecchia, A., and F. Sallusto. 2001. Regulation of t cell immunity by dendritic cells. Cell. 106:263–266.[CrossRef][Medline]
Lelouard, H., E. Gatti, F. Cappello, O. Gresser, V. Camosseto, and P. Pierre. 2002. Transient aggregation of ubiquitinated proteins during dendritic cell maturation. Nature. 417:177–182.[CrossRef][Medline]
Linder, S. 2007. The matrix corroded: podosomes and invadopodia in extracellular matrix degradation. Trends Cell Biol. 17:107–117.[CrossRef][Medline]
Linder, S., and M. Aepfelbacher. 2003. Podosomes: adhesion hot-spots of invasive cells. Trends Cell Biol. 13:376–385.[CrossRef][Medline]
Mott, J.D., and Z. Werb. 2004. Regulation of matrix biology by matrix metalloproteinases. Curr. Opin. Cell Biol. 16:558–564.[CrossRef][Medline]
Nakahara, H., L. Howard, E.W. Thompson, H. Sato, M. Seiki, Y. Yeh, and W.T. Chen. 1997. Transmembrane/cytoplasmic domain-mediated membrane type 1-matrix metalloprotease docking to invadopodia is required for cell invasion. Proc. Natl. Acad. Sci. USA. 94:7959–7964.
Nakamura, H., N. Suenaga, K. Taniwaki, H. Matsuki, K. Yonezawa, M. Fujii, Y. Okada, and M. Seiki. 2004. Constitutive and induced CD44 shedding by ADAM-like proteases and membrane-type 1 matrix metalloproteinase. Cancer Res. 64:876–882.
Poltorak, A., X. He, I. Smirnova, M.Y. Liu, C. Van Huffel, X. Du, D. Birdwell, E. Alejos, M. Silva, C. Galanos, et al. 1998. Defective LPS signaling in C3H/HeJ and C57BL/10ScCr mice: mutations in Tlr4 gene. Science. 282:2085–2088.
Ratzinger, G., P. Stoitzner, S. Ebner, M.B. Lutz, G.T. Layton, C. Rainer, R.M. Senior, J.M. Shipley, P. Fritsch, G. Schuler, and N. Romani. 2002. Matrix metalloproteinases 9 and 2 are necessary for the migration of Langerhans cells and dermal dendritic cells from human and murine skin. J. Immunol. 168:4361–4371.
Reis e Sousa, C. 2006. Dendritic cells in a mature age. Nat. Rev. Immunol. 6:476–483.[CrossRef][Medline]
Ridley, A.J., M.A. Schwartz, K. Burridge, R.A. Firtel, M.H. Ginsberg, G. Borisy, J.T. Parsons, and A.R. Horwitz. 2003. Cell migration: integrating signals from front to back. Science. 302:1704–1709.
Rousseau, S., M. Papoutsopoulou, A. Symons, D. Cook, J.M. Lucocq, A.R. Prescott, A. O'Garra, S.C. Ley, and P. Cohen. 2008. TPL2-mediated activation of ERK1 and ERK2 regulates the processing of pre-TNF alpha in LPS-stimulated macrophages. J. Cell Sci. 121:149–154.
Sallusto, F., and A. Lanzavecchia. 2000. Understanding dendritic cell and T-lymphocyte traffic through the analysis of chemokine receptor expression. Immunol. Rev. 177:134–140.[CrossRef][Medline]
Saltel, F., A. Chabadel, E. Bonnelye, and P. Jurdic. 2008. Actin cytoskeletal organisation in osteoclasts: A model to decipher transmigration and matrix degradation. Eur. J. Cell Biol. 87:459–468.[CrossRef][Medline]
Sato, H., T. Takino, and H. Miyamori. 2005. Roles of membrane-type matrix metalloproteinase-1 in tumor invasion and metastasis. Cancer Sci. 96:212–217.[CrossRef][Medline]
Schlondorff, J., and C.P. Blobel. 1999. Metalloprotease-disintegrins: modular proteins capable of promoting cell-cell interactions and triggering signals by protein-ectodomain shedding. J. Cell Sci. 112:3603–3617.[Abstract]
Seals, D.F., and S.A. Courtneidge. 2003. The ADAMs family of metalloproteases: multidomain proteins with multiple functions. Genes Dev. 17:7–30.
Shin, J.S., M. Ebersold, M. Pypaert, L. Delamarre, A. Hartley, and I. Mellman. 2006. Surface expression of MHC class II in dendritic cells is controlled by regulated ubiquitination. Nature. 444:115–118.[CrossRef][Medline]
Soond, S.M., B. Everson, D.W. Riches, and G. Murphy. 2005. ERK-mediated phosphorylation of Thr735 in TNFalpha-converting enzyme and its potential role in TACE protein trafficking. J. Cell Sci. 118:2371–2380.
Steinman, R.M., D. Hawiger, and M.C. Nussenzweig. 2003. Tolerogenic dendritic cells. Annu. Rev. Immunol. 21:685–711.[CrossRef][Medline]
Svensson, H.G., M.A. West, P. Mollahan, A.R. Prescott, R. Zaru, and C. Watts. 2008. A role for ARF6 in dendritic cell podosome formation and migration. Eur. J. Immunol. 38:818–828.[CrossRef][Medline]
Trombetta, E.S., and I. Mellman. 2005. Cell biology of antigen processing in vitro and in vivo. Annu. Rev. Immunol. 23:975–1028.[CrossRef][Medline]
Trombetta, E.S., M. Ebersold, W. Garrett, M. Pypaert, and I. Mellman. 2003. Activation of lysosomal function during dendritic cell maturation. Science. 299:1400–1403.
van Helden, S.F., D.J. Krooshoop, K.C. Broers, R.A. Raymakers, C.G. Figdor, and F.N. van Leeuwen. 2006. A critical role for prostaglandin E2 in podosome dissolution and induction of high-speed migration during dendritic cell maturation. J. Immunol. 177:1567–1574.
van Helden, S.F., M.M. Oud, B. Joosten, N. Peterse, C.G. Figdor, and F.N. van Leeuwen. 2008. PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis. J. Cell Sci. 121:1096–1106.
Van Lint, P., and C. Libert. 2007. Chemokine and cytokine processing by matrix metalloproteinases and its effect on leukocyte migration and inflammation. J. Leukoc. Biol. 82:1375–1381.
van Niel, G., R. Wubbolts, T. Ten Broeke, S.I. Buschow, F.A. Ossendorp, C.J. Melief, G. Raposo, B.W. van Balkom, and W. Stoorvogel. 2006. Dendritic cells regulate exposure of MHC class II at their plasma membrane by oligoubiquitination. Immunity. 25:885–894.[CrossRef][Medline]
Vu, T.H., J.M. Shipley, G. Bergers, J.E. Berger, J.A. Helms, D. Hanahan, S.D. Shapiro, R.M. Senior, and Z. Werb. 1998. MMP-9/gelatinase B is a key regulator of growth plate angiogenesis and apoptosis of hypertrophic chondrocytes. Cell. 93:411–422.[CrossRef][Medline]
Watts, C., R. Zaru, A.R. Prescott, R.P. Wallin, and M.A. West. 2007. Proximal effects of Toll-like receptor activation in dendritic cells. Curr. Opin. Immunol. 19:73–78.[CrossRef][Medline]
West, M.A., R.P. Wallin, S.P. Matthews, H.G. Svensson, R. Zaru, H.G. Ljunggren, A.R. Prescott, and C. Watts. 2004. Enhanced dendritic cell antigen capture via toll-like receptor-induced actin remodeling. Science. 305:1153–1157.
Wheeler, A.P., C.M. Wells, S.D. Smith, F.M. Vega, R.B. Henderson, V.L. Tybulewicz, and A.J. Ridley. 2006. Rac1 and Rac2 regulate macrophage morphology but are not essential for migration. J. Cell Sci. 119:2749–2757.
Wilson, N.S., and J.A. Villadangos. 2005. Regulation of antigen presentation and cross-presentation in the dendritic cell network: facts, hypothesis, and immunological implications. Adv. Immunol. 86:241–305.[CrossRef][Medline]
Zhou, Z., S.S. Apte, R. Soininen, R. Cao, G.Y. Baaklini, R.W. Rauser, J. Wang, Y. Cao, and K. Tryggvason. 2000. Impaired endochondral ossification and angiogenesis in mice deficient in membrane-type matrix metalloproteinase I. Proc. Natl. Acad. Sci. USA. 97:4052–4057.
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