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Membrane proteins stick around

Hitched to lipids, glowing proteins advance into a bleached area of the cell membrane.

You wouldn't call it a long-term relationship, but the interaction between proteins and certain membrane lipids is not just a one-millisecond stand. As Hammond et al. discovered, the proteins remain attached to the lipids longer than researchers expected. The findings explain how these lipids localize their effects.

By temporarily hooking up with various proteins, inositol lipids in the plasma membrane help cells do everything from transmit signals to move. The lipids can confine their influence to a specific section of the membrane. For example, a crawling cell cranks up inositol levels at its leading edge and destroys the lipids at its tail end. But inositol-bound proteins face a dilemma. As the inositols wander around the cell membrane, the proteins need to hang on long enough to encounter other proteins and thereby transmit the inositol signal. But if the proteins cling on too long, they risk spreading their effects beyond the target area. How the proteins resolve this dilemma wasn't clear because nobody had measured how long the molecules stay attached.

Using photobleaching, Hammond et al. estimated how fast protein–lipid couples moved within the membrane and how long they remained together. Riding the inositol molecules, the proteins traveled at a brisk pace of around 1 µm² per second, about the same speed as other researchers had measured for the lipids alone. To the team's surprise, the proteins could stick to the inositols for several seconds. As a result, a protein–inositol duo has time to search its neighborhood for potential interaction partners, but it can spread its influence over a few microns at the most. The researchers are now working to determine how interactions with other proteins affect the mobility of the pairs.

References

Hammond, G.R., et al. 2009. J. Cell Biol. doi:10.1083/jcb.200809073.[Abstract/Free Full Text]

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Reversible binding and rapid diffusion of proteins in complex with inositol lipids serves to coordinate free movement with spatial information

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This Article Full Text (PDF, 665K) PPT slides of all figures Alert me when this article is cited Services Email this article Similar articles in this journal Similar articles in PubMed Alert me to new content in the JCB Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Leslie, M. Search for Related Content PubMed PubMed Citation Articles by Leslie, M. Related Collections Related Article Social Bookmarking                            What's this?