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Antagonism between Smad1 and Smad2 signaling determines the site of distal visceral endoderm formation in the mouse embryo
Correspondence to Masamichi Yamamoto: myamamoto{at}fbs.osaka-u.ac.jp; or Hiroshi Hamada: hamada{at}fbs.osaka-u.ac.jp
The anterior–posterior axis of the mouse embryo is established by formation of distal visceral endoderm (DVE) and its subsequent migration. The precise mechanism of DVE formation has remained unknown, however. Here we show that bone morphogenetic protein (BMP) signaling plays dual roles in DVE formation. BMP signaling is required at an early stage for differentiation of the primitive endoderm into the embryonic visceral endoderm (VE), whereas it inhibits DVE formation, restricting it to the distal region, at a later stage. A Smad2-activating factor such as Activin also contributes to DVE formation by generating a region of VE positive for the Smad2 signal and negative for Smad1 signal. DVE is thus formed at the distal end of the embryo, the only region of VE negative for the Smad1 signal and positive for Smad2 signal. An inverse relation between the level of phosphorylated Smad1 and that of phosphorylated Smad2 in VE suggests an involvement of antagonism between Smad1- and Smad2-mediated signaling.
Abbreviations used in this paper: A-P, anterior–posterior; AVE, anterior visceral endoderm; BMP, bone morphogenetic protein; DVE, distal visceral endoderm; E, embryonic day; ES, embryonic stem; ExE, extraembryonic ectoderm; p-Smad, phosphorylated Smad; VE, visceral endoderm.
© 2009 Yamamoto et al.
This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.jcb.org/misc/terms.shtml). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
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Nodal, a secreted member of the TGF-β superfamily of ligands (Zhou et al., 1993), is required for DVE formation. ALK4 and ALK7 function as type 1 receptors for Nodal, whereas ActR2A and ActR2B function as type 2 receptors for this ligand. Nodal signaling is modulated by members of the EGF-CFC protein family and it is transduced by intracellular molecules including Smad2 and Smad3. With regard to formation of the A-P axis, Nodal–/– embryos manifest a failure of DVE formation at E5.5 (Brennan et al., 2001). However, a recent study (Mesnard et al., 2006) showed that the visceral endoderm (VE) of Nodal–/– embryos is abnormally specified before DVE formation. The primary role of Nodal in DVE formation is therefore to define an embryonic VE compartment before DVE formation.
The ExE is the source of another signal that regulates DVE formation (Rodriguez et al., 2005). Embryonic explants that lack ExE generate ectopic DVE, suggesting that a signal derived from ExE inhibits DVE formation (Rodriguez et al., 2005; Mesnard et al., 2006).
Bone morphogenetic proteins (BMPs) are also secreted ligands of the TGF-β superfamily and potential regulators of A-P patterning. BMPs function by binding to ALK1, ALK2, ALK3, or ALK6 as a type 1 receptor or to BMPR2, ActR2A, or ActR2B as a type 2 receptor (Zhao, 2003; Kishigami and Mishina, 2005). Gene targeting in the mouse has shown that BMP signaling is required for mesoderm formation (Mishina et al., 1995, 1999; Gu et al., 1999; Beppu et al., 2000; Lechleider et al., 2001; Tremblay et al., 2001). In the mouse embryo, anterior identity is established in the extraembryonic endoderm before formation of the primitive streak (Thomas et al., 1998; Beddington and Robertson, 1999). Some mutants that show a defect in mesoderm formation also manifest defective anterior patterning (Conlon et al., 1994; Brennan et al., 2001; Mesnard et al., 2006). Indeed, depletion of BMP4 has been shown to affect AVE formation (Soares et al., 2005). These various observations thus suggest that BMP signaling might regulate formation of the A-P axis, and that of DVE in particular.
To investigate the mechanism of DVE formation, we have now examined mutant mice that lack BMPR2, ActR2B, Lefty1, or Nodal and performed experiments with embryonic explants. Our data demonstrate that DVE formation is regulated by an antagonism between BMP-Smad1 and Activin/Nodal-Smad2 signaling. The DVE is thus formed at the distal region of the embryo where Smad2-mediated signal is present and Smad1-mediated signal is absent.
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Bmpr2–/– tetraploid chimeric embryos, which were generated by aggregation of ES cells expressing EGFP with Bmpr2–/– tetraploid embryos. In such chimeras, expression of Hex (n = 3) and Lefty1 (n = 3) was absent at E6.5 (Fig. 1, F–H and F'–H'). This phenotype was indistinguishable from that of Bmpr2–/– embryos, suggesting that BMPR2 in the extraembryonic region is required for DVE formation. We next examined whether VE is formed normally in Bmpr2–/– embryos. VE, which is composed of embryonic VE and extraembryonic VE at E5.5, is derived from the primitive endoderm of the E4.0–4.5 embryo. Expression of Hex, which is a marker of the primitive endoderm, was maintained in Bmpr2–/– embryos at E4.5 (n = 3; Fig. 1, I and I'). Expression of Hnf4 (n = 13), Gata4 (n = 4), and Pem (n = 7) was maintained in the extraembryonic VE of Bmpr2–/– embryos at E5.5 (Fig. 1, J' and K'; and Fig. S2 O'). However, expression of Bmp2 (n = 4), Fgf8 (n = 7), and Hnf4 (n = 13) in the embryonic VE was down-regulated in the mutant embryos at E5.2 and E5.5 (Fig. 1, J, J', L, L', M, M', and M"). Staining for phosphorylated ERK and expression of the ExE marker genes Eomes, Bmp4, and Mash2 were normal, whereas that of Wnt7b was slightly decreased, in the mutant embryos (Fig. S2, Q–W and Q'–W'). These results suggested that BMPR2 is not essential for formation of the primitive endoderm or extraembryonic VE, but rather is specifically required for specification of embryonic VE. The failure of DVE formation in Bmpr2–/– embryos is thus likely caused by the impaired differentiation of the primitive endoderm into embryonic VE.
Smad1-mediated signaling is reduced in Bmpr2–/– embryos
To evaluate how the lack of BMPR2 affects BMP signaling in the embryo, we examined the distribution of phosphorylated Smad1/5 (p-Smad1) in Bmpr2–/– embryos by immunohistofluorescence staining. In wild-type embryos, p-Smad1 was found in the nuclei of epiblast and primitive endoderm cells at E4.5 and of epiblast and VE cells at E5.2 (Fig. 1 N and Fig. 2, A and B). The distribution of p-Smad1 changed quickly between E5.2 and E5.5 and had shifted to the proximal epiblast and VE, excluding DVE, at E5.5 (Fig. 2 C and Fig. S3, A–J, available at http://www.jcb.org/cgi/content/full/jcb.200808044/DC1). In Bmpr2–/– embryos, the distribution of p-Smad1 was similar to that in wild-type embryos at E4.5 but showed two distinct patterns at later stages (Fig. 1 N and Fig. 2, A'–C'). In severely affected mutant embryos (8/14 embryos at E5.2 and 8/15 embryos at E5.5), p-Smad1 was apparent only in the proximal VE at E5.2 (8/8 embryos; Fig. 2 B') and was barely detected at E5.5 (8/8 embryos; Fig. 2 C'). In mildly affected embryos (6/14 embryos at E5.5 and 7/15 embryos at E5.5), p-Smad1 was found in the same regions as in wild-type embryos at E5.2, but its abundance was lower than that in the wild type (6/6 embryos; Fig. 1 N and Fig. S2). It was not detected in the epiblast and there were fewer positive cells in the VE of the mildly affected embryos at E5.5 (7/7 embryos; Fig. S2). These data suggested that BMP signaling is not completely lost but is reduced in Bmpr2–/– embryos. Other type 2 receptors may thus play a redundant role in this mutant. Variability in the phenotype of Bmpr2–/– embryos is most likely caused by the variable level of BMP signaling that remains.
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Bmpr2–/–,Actr2b+/– tetraploid chimeric embryos (Fig. S3, K and L). These results suggested that both BMPR2 and ActR2b act as receptors for BMP in VE. We next examined DVE markers in Bmpr2–/–,Actr2b+/– embryos at E5.5 to determine whether BMP signaling is required for DVE formation. Expression of DVE markers was detected in some of the Bmpr2–/– embryos examined (Fig. S2). However, expression of Lefty1 (n = 5), Cerl (n = 4), Dkk1 (n = 3), Lim1 (n = 4), and Hex (n = 3) was always lost in Bmpr2–/–,Actr2b+/– embryos (Fig. 3, I–M and I'–M'). We also examined endoderm markers in Bmpr2–/–,Actr2b+/– embryos from E4.5 to E5.5. Hnf4 (n = 5) and Pem (n = 3) were expressed normally at E5.5 (Fig. 3, N, N', O, and O'), whereas expression of Bmp2 (n = 4) and Fgf8 (n = 4) was abolished at E5.2 (Fig. 3, P, P', Q, and Q'), in the double mutant embryos. At E4.5, expression of Hex (n = 3) and Lefty1 (n = 4) was maintained (Fig. 3, R, R', S, and S'), suggesting that the primitive endoderm is correctly formed at E4.5 in the double mutant embryos. We then examined the expression of genes for Nodal signaling components and the distribution of phosphorylated Smad2/3 (p-Smad2) in Bmpr2–/–,Actr2b+/– embryos (Whitman, 2001). Nodal, Foxh1, and Cripto (n = 7, 4, and 4, respectively) were all expressed normally in the double mutant embryos at E5.5 (Fig. 3, T–V and T'–V'). The distribution of p-Smad2 in the double mutant embryos remained similar to that in wild-type or Bmpr2–/– embryos at E5.5 (Fig. 3, W, W', and X). Unlike Bmpr2–/–,Actr2b+/– embryos in which expression of DVE markers was always absent, it was detected in some of the Bmpr2–/–,Nodal+/– embryos examined (Fig. S4, A–M, available at http://www.jcb.org/cgi/content/full/jcb.200808044/DC1). Furthermore, p-Smad1 and p-Smad2 staining patterns of the Bmpr2–/–,Nodal+/– embryos were similar to those of the Bmpr2–/– embryos (Fig. S4, N–P). These results (summarized in Fig. 1 N) thus indicated that both BMPR2 and ActR2B function as type 2 receptors for BMP signaling during formation of embryonic VE and DVE.
Smad1 signaling is absent when and where DVE is newly formed
Staining for p-Smad1 was apparent throughout the primitive endoderm and VE until E5.2 (Fig. 2, A and B). At E5.5, however, p-Smad1 had disappeared from DVE while it was still apparent in the proximal portion of VE (Fig. 2 C and Fig. S3). In Lefty1–/– embryos (n = 5), the region of VE negative for p-Smad1 expanded (Fig. 4, A and C) along with the expansion of the region positive for DVE markers (Fig. 4, E–H). Thus, p-Smad1 is lost when and where DVE is formed.
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90 min after removal of ExE, whereas p-Smad1 staining is lost from VE much earlier, 30 min after ExE removal (Fig. 5, C–E, K, and L). These results suggested that loss of the BMP signal from prospective DVE, which coincides with the onset of DVE formation, may be required for DVE formation.
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To examine whether the ExE-derived inhibitory signal is indeed the BMP signal, we examined the response of DVE to BMP or a BMP inhibitor. When wild-type embryos were cultured from E5.2 to E5.5 in the presence of BMP4, expression of Hex, Lefty1, and Cerl was lost (Fig. S5, A–F, available at http://www.jcb.org/cgi/content/full/jcb.200808044/DC1). Whereas staining for p-Smad1 was lost in VE in response to removal of ExE at E5.5 (Fig. 6 A), p-Smad1 staining was maintained in VE if the explants lacking the ExE were cultured with BMP4 (Fig. 6 C). Moreover, expansion of the DVE region in such explants was inhibited by BMP4 (Fig. 6, B and D; and Fig. S5, T–W). In contrast, the region positive for DVE markers expanded and the level of expression of DVE markers increased when whole embryos at E5.2 were cultured with Noggin (Fig. 6, E–J), which induced down-regulation of p-Smad1 (Fig. 6, K and L).
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Antagonism between Smad2- and Smad1-mediated signaling in VE
A recent study showed that induction of DVE depends on Nodal and proprotein convertase activities (Mesnard et al., 2006). To detect Nodal activity in DVE, we first examined the distribution of p-Smad2 in wild-type embryos at E5.5. Staining for p-Smad2 was apparent in the epiblast (especially on the distal side) and VE including DVE (Fig. 3 W, Fig. 4 B, and Fig. 7 A). We also examined the distribution of p-Smad2 in Nodal–/– embryos at E5.5. Unexpectedly, however, staining for p-Smad2 was still apparent in the VE, including DVE, of these mutant embryos (Fig. 7, B and C), suggesting that the appearance of p-Smad2 in DVE is induced by another TGF-β–related factor. Activin is the most likely candidate because Activin-βA and Activin-βB are expressed in the decidual zone of the dam, and recombinant Activin is able to induce ectopic DVE (Jones et al., 2006; Mesnard et al., 2006). GDF1 and GDF3 may be other candidates that trigger Smad2-mediated signaling because DVE formation is impaired in a portion of the mutant mice lacking Gdf3 or Gdf3 and Gdf1 (Chen et al., 2006; Andersson et al., 2007). However, recent evidence (Andersson et al., 2007; Tanaka et al., 2007) has shown that the native form of GDF1 and GDF3 is incapable of inducing downstream signaling. GDF1 and GDF3 act as coligands of Nodal, which interact with Nodal and stimulate Nodal activity (Tanaka et al., 2007).
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Antagonism between Smad1 and Smad2 signaling is also apparent in the mutant embryo lacking Lefty1, an inhibitor of Nodal signaling. The distribution of p-Smad2 in Lefty1+/– embryos at E5.5 was similar to that in the wild type (unpublished data). However, the level of p-Smad2 was increased on the distal side of Lefty1–/– embryos at this stage (n = 5; Fig. 4, B and D). In contrast, the level of p-Smad1 was decreased on the distal side of Lefty1–/– embryos (n = 5; Fig. 4, A and C). In addition, the expression domains of Hex (n = 5) and Cerl (n = 5) were markedly expanded in the distal region of Lefty1–/– embryos (Fig. 4, E–H).
ActR2A and ActR2B are rate-limiting factors involved in the antagonistic balance
We next examined the molecular basis of the antagonism between BMP and Activin–Nodal signaling. Activin–Nodal and BMP share common signaling components, extracellular components such as ActR2A and ActR2B as well as intracellular components including Smad4. If the amount of a common component is limited, an increase in one signal would result in a decrease in the other. To test this possibility, we introduced a Smad4 or Actr2a+Actr2b expression vector into a p-Smad1–positive cell in the distal region of E5.2 embryo and examined if such cells would maintain or lose p-Smad1 12 h later (equivalent to E5.7; Fig. 8). Normally, such p-Smad1–positive cells at E5.2 (Fig. 2 B) lose p-Smad1 by E5.5 (Fig. 2 C). However, if a limiting factor commonly used for both signaling is overexpressed into such a cell, it would prevent the antagonism and the cell would remain positive for p-Smad1 at E5.5. This is what we observed when Actr2 was overexpressed (Fig. 8 A).
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At E5.5, DVE cells arise from a portion of embryonic VE that lacks the BMP signal. Whereas embryonic VE cells that have lost the BMP signal appear to be induced to differentiate into DVE, extraembryonic VE is not competent to become DVE even if it loses the BMP signal. First, in wild-type embryos at E5.2, before DVE formation, the entire embryonic VE is positive for the BMP signal. At E5.5, however, the BMP signal is lost specifically from DVE, with the remainder of the embryonic VE remaining positive for this signal (Fig. 2). Second, in Lefty1–/– embryos, the BMP-negative region of embryonic VE expands to the proximal side, toward which DVE formation also expands (Fig. 4). Third, the portion of embryonic VE that is converted to DVE was found to be increased in Noggin-treated embryos (Fig. 6). Although the BMP signal disappeared in both embryonic VE and extraembryonic VE of such embryos, extraembryonic VE was not converted to DVE. Fourth, in embryo explants stripped of ExE, in which Bmp4 and Bmp8b are expressed (Coucouvanis and Martin, 1999; Ying et al., 2000), the loss of BMP signaling precedes DVE expansion, suggesting that the former may be responsible for the latter. Expansion of Hex expression thus begins 90 min after the removal of ExE, whereas the BMP signal is lost from VE much earlier, 30 min after ExE removal (Fig. 5). Finally, in ExE-stripped embryos treated with BMP4 (Fig. 6) or with knockout serum replacement that contains BMP activity (Georgiades and Rossant, 2006), the Hex-expressing domain fails to expand. These results indicate that loss of the BMP signal from embryonic VE results in its conversion to DVE.
How is the BMP/Smad1 signal down-regulated in the region of DVE formation?
Although it is not clear how BMP signaling is down-regulated in the DVE region at E5.5, several mechanisms are possible. First, a BMP antagonist (or antagonists), such as Cerl, may be responsible. However, p-Smad1 was also absent in DVE of Cerl–/– embryos (Fig. 5 H), suggesting that Cerl is either not involved in or alone is not sufficient for the down-regulation of BMP signaling. Second, Bmp4 and Bmp8b, which encode the major BMP ligands at this stage, are expressed in ExE (Coucouvanis and Martin, 1999; Ying et al., 2000); elongation of the embryo at the egg cylinder stage along the proximodistal axis between E5.2 and E5.5 may therefore prevent BMPs from reaching the distal end of the embryo. Also, a low level of Bmp2 expression that can be detected in the distal region of the E5.2 embryo disappears by E5.5 (Fig. S5, I and J). Finally, an antagonism between Smad2 and Smad1 signaling may play the major role in down-regulation of the BMP signal. An inverse relation between the level of p-Smad1 and p-Smad2 was thus repeatedly observed in E5.2–5.5 embryos. In the wild-type embryo at E5.5, for instance, BMP signaling via p-Smad1 was detected predominantly in the proximal epiblast, a distribution opposite to that of p-Smad2 (Fig. 2 C vs. Fig. 4 B; and Fig. 7, A vs. J and D vs. G). Furthermore, p-Smad1 was lost from the distal half of VE in E5.5 Lefty1–/– embryos (Fig. 4 C) and Activin-treated wild-type embryos (Fig. 7 L), whereas the level of p-Smad2 was increased in this region. Staining for p-Smad1 was also lost from the entire VE, whereas that for p-Smad2 was increased in embryo explants stripped of ExE (Fig. 5) and in Noggin-treated embryos (Fig. 6, L and N). Staining for p-Smad2 was completely lost, whereas that for p-Smad1 was increased in embryos treated with SB431542 (Fig. S5, K–N). The antagonism between Smad1 and Smad2 signaling may convert a small shift of the balance to a robust difference.
Antagonism between Smad1 and Smad2 signaling
Our data suggest a previously unknown role for Activin in mouse embryonic patterning. Inhibition of Activin action by follistatin (Fig. 7, P–R) or SB431542 (Fig. S5, P and R) thus abolished DVE formation. Such treatment not only down-regulated p-Smad2 in DVE but also slightly up-regulated p-Smad1 on the distal side of the embryo. Conversely, treatment with Activin expanded the site of DVE formation by up-regulating p-Smad2 and down-regulating p-Smad1 on the distal side of the embryo (Fig. 7). These observations are again indicative of an antagonism between Smad1 and Smad2 signaling during DVE formation. Treatment of explants lacking ExE with SB431542 induced the loss of both p-Smad1 and p-Smad2. DVE formation was abolished in such explants (Mesnard et al., 2006; this study), suggesting that DVE formation requires both the absence of Smad1 signal and the presence of Smad2 signal. The balance between the ratio of p-Smad1 and p-Smad2 may determine whether or not a cell in the VE will become DVE. To test this, it would be necessary to quantify the amounts of p-Smad1 and p-Smad2 in individual cells and to monitor their interaction with Smad4, such as by FRET analysis.
Mutant embryos lacking Activin establish a normal A-P axis, most likely because Activin is provided by the decidual zone of the dam, not by the embryo (Albano et al., 1994; Jones et al., 2006). Maternally derived molecules of a large molecular weight can cross Reichert membrane and be transferred to the embryo. For example, maternal immunoglobulins (Bernard et al., 1977) and maternal and DiI-labeled high density lipoprotein intravenously injected into a pregnant mother (Smith et al., 2006) have been found in the embryo. In fact, maternally derived Activin proteins have been detected within the embryo including the VE between E3.5 and E6.5 (Jones et al., 2006).
Antagonism between Smad2 and Smad1 signaling may play a role in vertebrate development more generally. For example, BMP4 blocks the dorsal mesoderm-inducing activity of Activin in Xenopus laevis (Dale et al., 1992; Jones et al., 1992; Piccolo et al., 1996; Zimmerman and Mathews, 1996). Also in Xenopus, Chordin and Noggin prevent BMPs from interacting with their cognate receptors and thus allow underlying Activin signaling in the dorsal region of the embryo to induce dorsal mesoderm (Piccolo et al., 1996; Zimmerman and Mathews, 1996). Expression of Nodal is observed in the deep region of the involuting marginal zone, which is equivalent to AVE in mouse, before the onset of gastrulation in Xenopus embryos (Jones et al., 1995). Whereas p-Smad2 is present in these cells, p-Smad1 is absent (Schohl and Fagotto, 2002). In the mouse, Chordin and Noggin, in addition to Nodal, are expressed in the future node region (Conlon et al., 1994; Bachiller et al., 2000), and they may similarly shift the balance toward Activin–Nodal signaling. Indeed, the reduction in the level of Nodal signaling in Foxh1–/– embryos prevents node formation (Hoodless et al., 2001; Yamamoto et al., 2001). These observations suggest that the antagonism between Activin–Nodal and BMP signaling may play a role in organizer formation in vertebrates.
What is the molecular basis of the antagonism between BMP and Activin–Nodal signaling? Our result suggests that common signaling components, ActR2a and ActR2B, are rate-limiting factors in the E5.5 mouse embryo (Fig. 8). However, these results obtained by overexpression experiments need to be confirmed by other approaches because overexpressed type II receptor molecules may bind to type I receptor easily, which is often sufficient to activate signaling, or may interact with nonphysiological type I receptors. In Xenopus animal caps, Activin and BMP signaling have been shown to antagonize each other through the intracellular component Smad4 (Candia et al., 1997). However, the level of Smad4 was not low enough to cause the antagonism at least in the E5.5 mouse embryo. Different mechanisms may underlie the antagonism between BMP and Activin–Nodal depending on the cell type.
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Generation and analysis of chimeric embryos
Chimeras were generated by ES cell tetraploid embryo aggregation. Two-cell embryos were collected from intercrosses between Bmpr2+/– or Bmpr2+/–,Actr2b+/– mice and were aggregated with green ES FM260 cells expressing EGFP (provided by M. Okabe, Osaka University, Osaka, Japan). Chimeric embryos were recovered at the indicated stages, fixed, and both examined by fluorescence microscopy (MZ FLIII; Leica) using PlanApo 1.0x WILD objective (Leica) and processed for whole-mount in situ hybridization or for immunohistofluorescence analysis. The genotype of the host embryo was determined retrospectively with extraembryonic tissue. Images were obtained with a digital camera (DP11; Olympus) and processed with Photoshop CS software.
Embryo explant culture
For explant cultures, the ExE and epiblast overlying embryonic VE were removed from ICR mouse (SLC) or Hex-Venus transgenic mouse embryos dissected at E5.5 with fine forceps and a tungsten needle (Takaoka et al., 2006). Chimeric explants were generated with the use of a fine glass needle (Drummond Scientific Co.). The explants were cultured for 6 h or the indicated times under a humidified atmosphere of 5% CO2 at 37°C in dishes or in gel matrix (Mebiol) containing DME supplemented with 75% rat serum. Noggin, BMP4, follistatin, Activin (R&D Systems), and SB431542 (Sigma-Aldrich) were used at concentrations of 500 ng/ml, 50 ng/ml, 1.2 µg/ml, 50 µg/ml, and 10 µmol/l, respectively. Images were analyzed using a laser scanning confocal microscope (LSM 510) mounted on an inverted microscope (Axiovert 100M) using Plan Apochromat 20x/0.75 NA and C-Apochromat 40x/1.2 NA objectives (Carl Zeiss, Inc). Images were processed with Photoshop CS software.
Introduction of expression vectors into the epiblast
Introduction of expression vectors (a GFP expression alone or with various effectors; Mizushima and Nagata, 1990) into the epiblast was performed as described by Yamamoto et al. (2004). Embryos were cultured for 12 h under a humidified atmosphere of 5% CO2 at 37°C in dishes containing DME supplemented with 75% rat serum.
Online supplemental material
Fig. S1 shows expression of DVE markers in the wild-type mouse embryos at E5.2 and E5.5. Fig. S2 shows phenotype of AVE, VE, and ExE in Bmpr2–/– embryos. Fig. S3 shows p-Smad1 staining in wild-type E5.5 embryos and in the chimeric embryo at E5.0. Fig. S4 shows phenotype of DVE in Bmpr2–/–,Nodal+/– embryos. Fig. S5 shows effects of SB431542, follistatin, and BMP4 on DVE formation. Online supplemental material is available at http://www.jcb.org/cgi/content/full/jcb.200808044/DC1.
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Acknowledgments |
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This work was supported by a grant from Core Research for Evolutional Science and Technology of the Japan Science and Technology Corporation (to H. Hamada) as well as by a Grant-in-Aid for Scientific Research on Priority Areas and a Grant-in-Aid for Young Scientists (to M. Yamamoto) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. M. Yamamoto is also a recipient of a fellowship from the Japan Society for the Promotion of Science for Japanese Junior Scientists.
Submitted: 11 August 2008
Accepted: 22 December 2008
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