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Methylation of H3 K4 and K79 is not strictly dependent on H2B K123 ubiquitylation
Correspondence to Jessica A. Downs: j.a.downs{at}sussex.ac.uk
Covalent modifications of histone proteins have profound consequences on chromatin structure and function. Specific modification patterns constitute a code read by effector proteins. Studies from yeast found that H3 trimethylation at K4 and K79 is dependent on ubiquitylation of H2B K123, which is termed a "trans-tail pathway." In this study, we show that a strain unable to be ubiquitylated on H2B (K123R) is still proficient for H3 trimethylation at both K4 and K79, indicating that H3 methylation status is not solely dependent on H2B ubiquitylation. However, additional mutations in H2B result in loss of H3 methylation when combined with htb1-K123R. Consistent with this, we find that the original strain used to identify the trans-tail pathway has a genomic mutation that, when combined with H2B K123R, results in defective H3 methylation. Finally, we show that strains lacking the ubiquitin ligase Bre1 are defective for H3 methylation, suggesting that there is an additional Bre1 substrate that in combination with H2B K123 facilitates H3 methylation.
Abbreviation used in this paper: PIKK, phosphatidylinositol kinase–like kinase.
© 2009 Foster and Downs
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Between 1 and 10% of histone H2B in budding yeast is ubiquitylated on K123 (Robzyk et al., 2000). This is catalyzed by Rad6 and Bre1, which encode an E2 and E3 ubiquitin ligase, respectively (Robzyk et al., 2000; Hwang et al., 2003; Wood et al., 2003a). Previous studies found that disruption of RAD6 or BRE1 or mutation of H2B K123 results in a loss of H3 methylation at K4 and K79 (Briggs et al., 2002; Dover et al., 2002; Ng et al., 2002; Sun and Allis, 2002), and this has been termed a "trans-tail pathway" (Briggs et al., 2002; Henry et al., 2003). More recently, it appears that monomethylation of H3 K4 and K79 is unaffected by the absence of H2B ubiquitylation (Dehe et al., 2005). Although it is not clear whether dimethylation is dependent on H2B ubiquitylation, there is a consensus that this trans-tail pathway is important for trimethylation of these residues (Dehe et al., 2005; Laribee et al., 2005; Shahbazian et al., 2005; Shukla et al., 2006).
H3 K4 methylation is catalyzed by the Set1 methyltransferase and H3 K79 methylation by Dot1. In addition to effects on regulation of gene expression, these modifications contribute to DNA damage responses. dot1 or H3 K79E mutant strains are UV sensitive (Bostelman et al., 2007). Additionally, a dot1 mutant strain was found to be ionizing radiation sensitive in one study (Game et al., 2006) but only when combined with mutations in other methyltransferases, such as set1, in another (Wysocki et al., 2005). The dot1 and H3 K79A mutant strains were found to have a defect in DNA damage checkpoints (Giannattasio et al., 2005; Wysocki et al., 2005), and genetic analysis of the strains in response to UV irradiation suggests that there is also a contribution to survival after DNA damage beyond mediating checkpoint responses (Bostelman et al., 2007). Although the involvement of Set1-mediated methylation of H3 K4 in DNA damage responses is less well characterized, Set1 appears to contribute to the intra–S phase DNA damage checkpoint (Wysocki et al., 2005).
Consistent with a dependence on K123 ubiquitylation, rad6 and bre1 mutant strains are hypersensitive to ionizing radiation, function on the same pathway as dot1 (Game et al., 2006), and, like htb1-K123R mutant cells, show DNA damage checkpoint defects (Giannattasio et al., 2005; Wysocki et al., 2005).
One of the most well-characterized DNA damage–dependent histone modifications is the phosphorylation of histone H2A (H2AX in higher eukaryotes) by the phosphatidylinositol kinase–like kinases (PIKKs; Mec1 and Tel1 in budding yeast; Downs et al., 2007). The phosphorylated motif is a well-defined consensus site for this family of kinases (S/TQ), and its presence in histone H2A variants is well conserved throughout eukaryotic evolution (Foster and Downs, 2005). Despite the rapid and robust phosphorylation of H2A in response to DNA damage, mutation of the site results in a very mild hypersensitivity to DNA-damaging agents (Downs et al., 2000; Redon et al., 2003). We noted the existence of an additional PIKK consensus site in the C-terminal tail of H2B and considered that it may play a redundant role in mediating cellular responses to DNA damage.
In this study, we investigate the role of the C-terminal tail of H2B in DNA damage responses. We found that the PIKK consensus site does not contribute to DNA damage responses but that other mutations in the C-terminal tail result in hypersensitivity to DNA damage. In contrast to previous studies (Briggs et al., 2002; Dover et al., 2002; Ng et al., 2002; Sun and Allis, 2002; Henry et al., 2003), we also found that a strain in which H2B cannot be ubiquitylated (htb1-K123R) was not defective in H3 trimethylation at K4 and K79, indicating that H3 methylation is not strictly dependent on H2B ubiquitylation. Interestingly, we found that if the K123R mutation was combined with either mutation of two serines in the H2B tail or the addition of an N-terminal Flag tag (htb1-K123R/S125A/S126A or Flag–htb1-K123R), the resulting strains were both hypersensitive to DNA damage and lost methylation at H3 K4 and K79. Moreover, we found that the original strain used to elucidate the trans-tail pathway has a mutation in the genome that, when combined with htb1-K123R, results in defective H3 methylation. Together, these data demonstrate that H2B ubiquitylation contributes to normal H3 methylation but is not the sole determinant of this pathway.
Consistent with previous results (Briggs et al., 2002; Dover et al., 2002; Ng et al., 2002; Sun and Allis, 2002; Henry et al., 2003), however, we found that disruption of the Bre1 ubiquitin ligase resulted in both hypersensitivity to DNA damage and loss of H3 K4 and K79 trimethylation. This suggests that Bre1 may have an additional target that is critical for H3 methylation.
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Results and discussion |
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Next, we examined the ability of these strains to be ubiquitylated using N-terminally Flag-tagged constructs. We found that the slower migrating band corresponding to ubiquitylated H2B disappears in both the K123R and KSS mutant strains as expected. Mutation of the serines alone appears to have no effect on H2B ubiquitylation (Fig. 2 B).
Because H2B ubiquitylation had been implicated in DNA damage responses (Giannattasio et al., 2005), we compared our strains with those used in previous studies (Y133 and its parent strain Y131; Robzyk et al., 2000; Kao et al., 2004; Giannattasio et al., 2005; Shahbazian et al., 2005) and found that the htb1-K123R mutant in the Y133 strain background shows hypersensitivity to DNA damage relative to the Y131 parent strain background (Fig. 2 C). We also noted that although our htb1-K123R (K) and our htb1-S125A/S126A (SS) mutant strains grew as well as wild type (Fig. 2 F and not depicted), our KSS triple mutant displayed a slight slow growth phenotype (Fig. 2 D).
We also examined the phenotypes of our Flag-tagged constructs. Although the addition of the Flag tag to wild-type H2B did not affect the ability of the strain to survive when exposed to phleomycin, the Flag–htb1-K123R (Flag-K) mutant strain displayed a striking hypersensitivity (Fig. 2 E). Because the htb1-K123R parent strain was indistinguishable from wild type in this assay (Fig. 2 E), this suggests that the combination of the two alterations to the H2B gene results in impaired DNA damage responses. Interestingly, this is very similar to the phenotype seen when the K123R mutation is combined with the S125A/S126A mutations (KSS; Fig. 2 A), and we found that the Flag-KSS mutant strain was no more sensitive than the Flag-K mutant (Fig. 2 E). Moreover, the Flag-K mutant strain displays a similar slow growth phenotype to the KSS triple mutant (Fig. 2 F). Altogether, these data suggest that the addition of the Flag epitope to H2B may impair similar functions as the htb1-S125A/S126A mutations. These data also indicate that H2B K123 plays a role in DNA damage responses, but this contribution is only detectable when other H2B functions are impaired.
H3 trimethylation of K4 and K79 is not dependent on H2B ubiquitylation
As previously discussed, H3 trimethylation at K4 and K79 is dependent on H2B K123 ubiquitylation in strains derived from Y131 (Dehe et al., 2005; Laribee et al., 2005; Shahbazian et al., 2005; Shukla et al., 2006). We investigated these modifications in our H2B mutant strains. Strikingly, we found that H3 trimethylation of both K4 and K79 was indistinguishable from wild type in our K123R mutant strain (Fig. 3 A), indicating that ubiquitylation of H2B is not a prerequisite for these H3 methylation events. Consistent with previous studies, however, both K4 and K79 trimethylation was abolished in the Y133 background (K123R; Fig. 3 A; Dehe et al., 2005; Laribee et al., 2005; Shahbazian et al., 2005; Shukla et al., 2006). Interestingly, in our KSS triple mutant strain, which phenocopies the Y133-based htb1-K123R strain, we found that the trimethylation of both K4 and K79 was lost (Fig. 3 A), whereas no change was detected in the SS mutant strain. This is consistent with a lack of hypersensitivity to DNA damage in this strain. dot1 and set1 mutant strains were tested in parallel to demonstrate the specificity of the antibodies (Fig. 3 A).
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Next, we investigated the loss of H3 trimethylation in the Y133 (htb1-K123R) mutant strain background. We considered that perhaps differences in the H2B coding sequences present on the plasmids used in Y133 compared with FY406 accounted for the different phenotypes. We shuffled our htb1-K123R–encoding plasmid into the Y133 strain background, replacing the original htb1-K123R plasmid in this strain, and found that the resulting strain was still hypersensitive to phleomycin and still had defective H3 methylation (Fig. 3, C and D). Interestingly, however, when we shuffled our htb1-K123R mutant construct into the Y131-based parent strain, we found that similar to our strain background, this Y131-based htb1-K123R mutant strain was not hypersensitive to DNA damage and had normal H3 methylation (Fig. 3, C and D).
Because Y133 was created from Y131, once the H2B-encoding plasmids were replaced with our htb1-K123R construct, the resulting strains should be genetically identical. Therefore, one explanation for these results is that Y133 sustained a mutation in the genome that, when combined with the htb1-K123R mutation (regardless of which plasmid construct is used), results in the phenotypes seen here.
To test this, we performed a genetic cross between the Y131- and Y133-based strains. Tetrads were dissected, and the htb1-K123R mutant construct was shuffled into the haploid daughter strains. Hypersensitivity to phleomycin and defective H3 methylation segregated 2:2 (Fig. 4 and not depicted). In contrast, spores derived from Y131 crossed with itself as a control consistently showed no hypersensitivity to phleomycin and had normal H3 methylation when carrying the htb1-K123R mutation (Fig. 4 and not depicted). These data demonstrate that the defect in H3 trimethylation in Y133 is a product of the htb1-K123R mutation in combination with a mutation in the genome.
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We identified three different genetic changes that, in combination with htb1-K123R, result in defective H3 trimethylation. Serine to alanine substitutions of H2B S125 and S126 or the addition of an N-terminal Flag epitope can result in the loss of H3 trimethylation when combined with the htb1-K123R mutation. Although we do not know the change in Y133 responsible for the loss of H3 trimethylation, we can rule out changes in H2B because swapping the sole source of H2B makes no difference (Fig. 3, compare A [fifth and sixth lanes] with C [third and fourth lanes]). We are currently working to identify the nature of the mutation in Y133, as this will shed light on the mechanism of the trans-tail pathway. It is also worth emphasizing that the addition of an N-terminal Flag epitope is not a neutral event, and studies using strains with this construct must be interpreted with caution.
H3 trimethylation of K4 and K79 is dependent on the Bre1 ubiquitin ligase
Notably, multiple groups using different strain backgrounds found that strains lacking genes required for H2B ubiquitylation such as RAD6 and BRE1 are hypersensitive to DNA damage and lose H3 K4 and K79 methylation (Dover et al., 2002; Sun and Allis, 2002; Wood et al., 2003b; Laribee et al., 2005; Game et al., 2006). We created a bre1 mutant strain in W303 and found that, consistent with previous studies (Dover et al., 2002; Sun and Allis, 2002; Wood et al., 2003b; Laribee et al., 2005; Game et al., 2006), H3 K4 and K79 trimethylation is absent in this strain (Fig. 5 A).
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Because trimethylation is lost in the absence of Bre1, but not in the htb1-K123R mutant strain, this suggests that Bre1 has a contribution to the regulation of H3 methylation beyond the ubiquitylation of H2B K123. Notably, the absence of ubiquitin from a reconstituted in vitro transcription system results in the loss of H3 trimethylation of K4 (Pavri et al., 2006), suggesting that the putative second target is either another residue in the nucleosome or the methyltransferase itself. This is consistent with a recent study in which Bre1 was found to ubiquitylate the Swd2 subunit of COMPASS (complex proteins associated with Set1) to facilitate H3 K4 trimethylation (Vitaliano-Prunier et al., 2008). Additionally, H2B was recently found to be ubiquitylated at other sites, which may be important for H3 methylation (Geng and Tansey, 2008). Although the trans-tail pathway exists to regulate the methylation status of histone H3, it is clearly more complex than originally thought and requires activities beyond ubiquitylation of histone H2B at K123.
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Materials and methods |
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Western blot analyses
Midlog cultures were treated with or without 20 µg/ml phleomycin for 2 h unless otherwise indicated. Cell lysates were prepared using standard glass bead disruption into 20% TCA. Samples were electrophoresed on 15% SDS-PAGE, transferred to nitrocellulose, and analyzed with the following antibodies: 1:500 anti–Flag M2 (Sigma-Aldrich), 1:1,000 anti-H3 (Abcam), 1:2,000 anti-H3K4me3 (Millipore), 1:500 anti-H3K79me3 (Abcam), and 1:2,000 anti-H2B (generated against recombinant H2B). HRP-conjugated anti–mouse (Abcam) or anti–rabbit (Abcam) secondary antibodies were used, and bands were visualized by chemiluminescence (Thermo Fisher Scientific).
Genetic crosses
The Y131- and Y133-based strains (YM5037 and YM5038) were transformed with pJD150 (HTA1-HTB1, HIS3) and were 5-fluoroorotic acid selected. The mating type of YM5037 was switched, and this strain was crossed with YM5037 or YM5038 to create the diploid strains JDY771 and JDY772, respectively. The strains were grown in rich media, and single colonies were screened for Ura+ His– phenotypes, reflecting the sole presence of the URA3-based plasmid encoding wild-type H2B. These were sporulated, tetrads were dissected, and sets of four segregants were transformed with pJD173 (HTA1-htb1-K123R, HIS3) and were 5-fluoroorotic acid selected.
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Acknowledgments |
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E.R. Foster was supported by a studentship from the Biotechnology and Biological Sciences Research Council, and J.A. Downs was a Jenner Fellow of the Lister Institute.
Submitted: 15 December 2008
Accepted: 2 February 2009
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helix (
) or Set1 (set1
