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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200207101
Kruse et al.
Supplemental materials and methods
Figure S1 ASH1 mRNA localization is unchanged in cells expressing Myo4-GFP.
Figure S2 Myo2p localization and actin cables remain largely intact in mex67-5 cells at the nonpermissive temperature.
Figure S3 Myo4p-HA and ASH1 mRNA accumulation at bud tips is disrupted by treatment with the translational inhibitor cycloheximide.
Video 1 (892 K) Large Myo4-GFP particles in wild-type cells move along the bud cortex and frequently split into smaller particles but reassemble.
Video 2 (964 K) In she2
mutant cells, Myo4-GFP signal is weaker and its distribution is more diffuse.
Table S1 Oligonucleotides used in this study
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