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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200207101
Kruse et al.

Supplemental materials and methods

Figure S1 ASH1 mRNA localization is unchanged in cells expressing Myo4-GFP.

Figure S2 Myo2p localization and actin cables remain largely intact in mex67-5 cells at the nonpermissive temperature.

Figure S3 Myo4p-HA and ASH1 mRNA accumulation at bud tips is disrupted by treatment with the translational inhibitor cycloheximide.

Video 1 (892 K) Large Myo4-GFP particles in wild-type cells move along the bud cortex and frequently split into smaller particles but reassemble.

Video 2 (964 K) In she2 mutant cells, Myo4-GFP signal is weaker and its distribution is more diffuse.

Table S1 Oligonucleotides used in this study





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