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Video 1, A (5.0 MB) and
B (5.0 MB)
(A) Fibroblast-like, mesenchymal migration
of HT1080/MT1-MMP cells through 3D collagen
matrix (high magnification time lapse video recording).
Pseudopods at the leading edge generate transient
attachment to and localized pulling and
distortion of collagen fibers for migratory
force generation. The image size is 94 x 70 µm. The
time frame is in the bottom left corner (h:min:s).
(B) Blocking of ß1 integrins (mAb 4B4) results in
spherical morphology as a consequence of impaired collagen
fiber binding, indicating ß1 integrins as sole receptors
to fibrillar type I collagen in HT1080 cells.
Nonproductive ruffling is insufficient to generate
traction-dependent polarization and migration.
The image size is 70 x 70 µm. The time frame is
2 h, 50 min.
