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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200209022
Lee et al.

Figure S1 Growth of pac1{Delta} double mutant strains.

Figure S2 Function of PAC1-3GFP and DYN1-3GFP.

Figure S3 Immunoblot analysis of Dyn1-3GFP protein in wild-type and pac1{Delta} cells.

Figure S4 Phylogenetic analysis of LIS1 proteins.

Figure S5 Domain organization and coiled-coil structure prediction.

Video 1 (5.6 MB) A wild-type cell expressing GFP-labeled microtubules (GFP-TUB1).

Video 2 (8.2 MB) A pac1{Delta} cell expressing GFP-labeled microtubules (GFP-TUB1).

Video 3 (6.3 MB) A pac1{Delta} cell expressing GFP-labeled microtubules (GFP-TUB1).

Video 4 (128 K) Localization of Pac1-3GFP in a wild-type cell.

Video 5 (96 K) Localization of Pac1-3GFP in wild-type cells.

Video 6 (88 K) Pac1-3GFP in a wild-type cell.

Video 7 (436 K) Pac1-3GFP in a num1{Delta} cell.

Video 8 (1.0 MB) Pac1-3GFP in a nip100{Delta} cell.

Video 9 (664 K) Pac1-3GFP in dyn1{Delta} cells.

Video 10 (892 K) Localization of Dyn1-3GFP in a wild-type cell.

Video 11 (992 K) Dyn1-3GFP in wild-type cells.

Video 12 (304 K) Dyn1-3GFP in an arp1{Delta} cell.

Video 13 (304 K) Dyn1-3GFP in a num1{Delta} cell.

Video 14 (620 K) Dyn1-3GFP in pac1{Delta} cells.

Video 15 (6.8 MB) Dyn1-3GFP in pac1{Delta} cells.

Video 16 (1.3 MB) Proposed mechanism for microtubule sliding along the bud cortex.

 





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