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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200309101
Gómez-Moutón et al.
• Figure S1 GFP-GPI–expressing Jurkat cells were stained with biotinylated CTx (A) or anti-GM3 antiserum (B) as indicated.
• Figure S2 Single-color images of Jurkat cells expressing N or C terminus GFP-tagged CCR5 chimeras
• Video 1 (124 K) Redistribution of GFP-GPI in chemotaxing Jurkat cells.
• Video 2 (480 K) Redistribution of GFP-GPI in chemotaxing HL60 cells.
• Video 3 (260 K) Redistribution of GFP-GPI in chemotaxing HL60-DMSO cells.
• Video 4 (212 K) Nonraft GFP-GT46 membrane protein remains evenly distribute in chemotaxing Jurkat cells.
• Video 5 (624 K) Nonraft GFP-GT46 membrane protein remains evenly distributed GFP-GT46 in chemotaxing HL60 cells.
• Video 6 (244 K) Redistribution of GFP-GPI requires Gi protein signaling.
• Video 7 (264 K) Redistribution of GFP-GPI requires an intact actin cytoskeleton.
• Video 8 (528 K) CCR5 accumulates at the leading edge in polarized cells.
• Video 9 (760 K) PI3K is recruited to the leading edge in polarized cells.
• Video 10 (388 K) p110
leading edge accumulation is not shared by a coexpressed inert cytosolic marker.
• Video 11 (260 K) PI3K is activated to the leading edge in polarized cells.
• Video 12 (200 K) Inhibition of PI3K prevents polarized recruitment of the AKT PH domain.
• Video 13 (224 K) Depletion of plasma membrane cholesterol prevents cell polarization.
• Video 14 (596 K) Replenishment of membrane cholesterol restores polarization.
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