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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200402052
Inoue et al.

Table S1 Frequencies of division abnormalities in wild-type and orbit7 genotypes

Figure S1 Phase-contrast images of onion stage spermatids isolated from homozygous orbit11 and ß-tubulin-EGFP flies.

Figure S2 Selected frames from a time-lapse sequence showing MT behavior in an S2 cell expressing tubulin tagged with GFP during cytokinesis.

Video 1 (9.1 MB) Cytokinesis in a wild-type Drosophila primary spermatocyte expressing ß-tubulin-GFP.

Video 2 (1.2 MB) Peripheral MTs probe the cortex and become bundled at the future cleavage site.

Video 3 (1.1 MB) A video of a GFP tubulin-expressing S2 cell reveals that post anaphase astral MTs grow towards the cortex and contact the cleavage site almost simultaneously with furrow initiation.

Video 4 (7.4 MB) Time-lapse sequence of the orbit mutant cell.

Video 5 (8.7 MB) Another example of cytokinesis in an orbit mutant cell.





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