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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200403036
Powers et al.

• Table S1 Data from time-lapse confocal videos of C. elegans embryos that expressed GFP::histone (chromosomes) and GFP::-tubulin (poles)

• Figure S1 Comparisons of structural and sequence features of chicken chromokinesin, mouse Kif4, Drosophila Klp3A, C. elegans KLP-19, C. elegans KLP-12, and human Kid.

• Video 1 (4.8 MB) A time-lapse scanning confocal microscopy movie showing a single optical section of the AB (left) and P₁ (right) blastomeres of a wild-type two-cell embryo.

• Video 2 (5.1 MB) Time-lapse confocal microscopy movie showing a single optical section of mitotic chromosomes (GFP::histone) in the AB (left) and P₁ (right) blastomeres of a two-cell embryo subjected to klp-19 RNAi by injection.

• Video 3 (1.4 MB) Time-lapse confocal microscopy movie showing a single optical section of chromosomes (GFP::histone) undergoing their first mitosis in KLP-19-depleted cytoplasm.

• Video 4 (2.6 MB) Time-lapse confocal microscopy movies showing single optical sections of first mitosis in two different wild-type embryos containing GFP::histone and GFP::-tubulin, which show chromosomes and spindle poles.

• Video 5 (2.6 MB) Time-lapse confocal movies of first mitosis in two klp-19(RNAi) embryos containing GFP::histone and GFP::-tubulin.

• Video 6 (740 K) A time-lapse confocal movie of P1cell mitosis in an embryo expressing GFP::histone and GFP::-tubulin and treated with 20 µg/ml nocodazole to deplete microtubules.

• Video 7 (940 K) A time-lapse confocal movie of first mitosis in an embryo depleted of HCP-3 (CENP-A), which is required for assembly of mitotic kinetochores.

• Video 8 (3.4 MB) Time-lapse confocal movies of a bipolar mitosis in a P₀ cell followed by a monopolar mitosis in the AB daughter cell of a zyg-1 control embryo (top) and a zyg-1; klp-19(RNAi) embryo (bottom) containing GFP::histone and GFP::-tubulin.

• Video 9 (832 KMB) Time-lapse confocal movies of a monopolar mitosis in the AB cell of a zyg-1 control embryo (top) and a zyg-1; klp-19(RNAi) embryo (bottom) containing GFP::histone and GFP::-tubulin.

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