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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200407058
Golub et al.
• Figure S1 Cell surface rafts accumulate at motile lamellipods.
• Figure S2 Stabilization of microtubules at PI(4,5)P2-rich raft patches.
• Figure S3 Association of APC with cell edge raft patches.
• Video 1 (1.2 MB) Phase-contrast time-lapse recording of PC12 cells in the absence of NGF.
• Video 2 (3.3 MB) Phase-contrast time-lapse recording of NGF-treated PC12 cells.
• Video 3 (1.5 MB) Phase-contrast time-lapse recording of PC12 cell pretreated with cyclodextrin, and then with NGF.
• Video 4 (1.2 MB) GFP-fluorescence time-lapse recording of NGF-treated PC12 cell expressing PH1-GFP.
• Video 5 (2.5 MB) Time-lapse recording of NGF-treated PC12 cells labeled with DiD.
• Video 6 (1.0 MB) GFP-fluorescence time-lapse recording of NGF-treated PC12 cells coexpressing PH1-GFP and Dn-Cdc42.
• Video 7 (3.5 MB) Phase-contrast time-lapse recording of NGF-treated PC12 cells in the presence of nocodazole (added 5 min before NGF).
• Video 8 (1.0 MB) GFP-fluorescence time-lapse recording of NGF-treated PC12 cells expressing PH1-GFP in presence of nocodazole (added 5 min before NGF).
• Video 9 (1.0 MB) GFP-fluorescence time-lapse recording of NGF-treated PC12 cells expressing PH1-GFP in the presence of Sp-cAMP (added 30 min before NGF).
• Video 10 (1.0 MB) GFP-fluorescence time-lapse recording of NGF-treated PC12 cells expressing PH1-GFP in presence of Rp-cAMP (added 30 min before NGF).
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