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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200409058
Evans et al.

Figure S1 Hydrolysis of ATP by recombinant Spastin results in decreased microtubule content in permeabilized cells.

Figure S2 Binding of Spastin along the length of microtubules.

Video 1 (3.7 MB) Time-lapse microscopy of Spastin-mediated microtubule severing.

Video 2 (1.1 MB) Detailed view of time-lapse microscopy of Spastin-mediated microtubule severing.

Video 3 (3.0 MB) Time-lapse microscopy of a permeabilized cell incubated with E442Q Spastin.

Video 4 (3.6 MB) Time-lapse microscopy of Spastin-mediated severing of purified microtubules.

Video 5 (3.1 MB) E442Q Spastin does not sever microtubules.





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