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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200409099
Khaled et al.
• Figure S1 FL5.12A cells cultured with IL-3 incorporate BrdU indicating DNA synthesis is occurring, while withdrawal of IL-3 induces growth arrest demonstrated by decreased BrdU uptake as a result of decreased DNA synthesis.
• Figure S2 p38 MAPK activity increases upon cytokine withdrawal while Chk1 protein levels decrease.
• Figure S3 Overexpression of cyclin D1 does not mimic the effects of p38 MAPK inhibition on cell cycling during IL-3 withdrawal.
• Figure S4 Primary lymph node (LN) lymphocytes were cotransfected with Cdc25A-WT or Cdc25A-DP and GFP.
• Figure S5 Overexpression of BCL-XL protects from apoptosis due to IL-3 withdrawal but does not promote cell cycling and maintains cells in a small, vegetative state.
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