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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200409157
Fivaz et al.
• Figure S1 Distribution of lipid-modified fluorescent probes (described in Table S1) in hippocampal neurons (12-15 DIV).
• Figure S2 Glutamate-induced translocation of CFP-KRas-tail in BFA-treated hippocampal neurons.
• Figure S3 Examples of glutamate-induced recruitment of CaM to the PM in KRas-tail-expressing neurons.
• Figure S4 Examples showing that YFP-CaM or CFP-CaM is not recruited to the PM upon glutamate stimulation in cells expressing CFP-HRas-tail or YFP-HRas-tail.
• Figure S5 Relative expression levels of exogenous vs. native CaM.
• Table S1
• Video 1 (2.4 MB) Glutamate-induced translocation of CFP-KRas-tail to perinuclear membranes.
• Video 2 (2.3 MB) Transient application of glutamate leads to reversible translocation of CFP-KRas-tail.
• Video 3 (1.3 MB) CFP-KRas-tail translocates to early endosomes in dendritic branches.
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