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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200411001
Wood et al.

Figure S1 Uncaging cGMP increases the velocity of swimming sperm.

Figure S2 Uncaging cGMP in the absence of fluo-4 does not alter the motility response.

Figure S3 Schematic diagram showing arrangement of imaging equipment used in this study.

Video 1 (5.8 MB) Montage of typical untreated sperm (above) and 30 µM nimodipine-treated sperm (below) before and after UV flash.

Video 2 (4.2 MB) Effect of repetitive UV flashes on [Ca2+]i and trajectory of untreated caged cGMP-loaded sperm.

Video 3 (5.8 MB) Effect of repetitive UV flashes on [Ca2+]i and trajectory of 30 µM nimodipine-treated sperm loaded with caged cGMP.





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