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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200412032
Smith et al.
Figure S1 LFA-1 surface distribution on a T cell migrating in a SDF-1 chemotactic gradient.
Figure S2 Polarity markers and surface distribution on T cells migrating on ICAM-1.
Figure S3 A T cell migrating on ICAM-1 in the presence of mAb 24.
Figure S4 The sequence for the PIPKI
plus control peptide and the effect of the control peptide on the focal zone.
Video 1 (2.2 MB) Human T cells labeled with Alexa-546 nonfunction-blocking anti-LFA-1 Fab' plated onto TNF-treated HUVECs.
Video 2 (568 k) Location and stability of the focal zone on human T cells migrating on ICAM-1 embedded in the lipid bilayer.
Video 3 (2.8 MB) The attachment and migration of a T cell to ICAM-1 embedded in the lipid bilayer.
Video 4 (9.7 MB) PIPKI
peptides (30 µg/ml) effect on focal zone stability and migratory speed on human T cells migrating on ICAM-1 embedded in the lipid bilayer.
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