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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200501131
Bement et al.
• Figure S1 GFP-rGBD is a faithful reporter of RhoA activity in echinoderms.
• Figure S2 The RhoA zone is conserved in other species and cell types.
• Figure S3 Actin accumulation is not required for RhoA zone formation.
• Figure S4 The RhoA activity zone follows the spindle.
• Video 1 (1.1 MB) Accumulation of eGFP-rGBD in the urchin furrow.
• Video 2 (2.7 MB) Accumulation of eGFP-rGBD in the urchin furrow.
• Video 3 (2.4 MB) Accumulation of eGFP-rGBD in the X. laevis furrow.
• Video 4 (1.7 MB) RhoA activity during unequal cleavage (micromere formation) in the urchin embryo.
• Video 5 (180 k) RhoA activity during polar body emission in X. laevis.
• Video 6 (1.3 MB) The RhoA zone forms in the presence of cytochalasin D.
• Video 7 (1.1 MB) RhoA activity after extreme disruption of the cortex by cytochalasin D.
• Video 8 (1.6 MB) Isolation of the cortex from the spindle prevents focusing of the RhoA activity zone.
• Video 9 (816 k) The RhoA activity zone moves after displacement of the spindle.
• Video 10 (1.0 MB) Half spindles induce RhoA activation.
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