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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200504029
Nishita et al.
• Figure S1 Changes in the total F-actin content in Jurkat cells before and after SDF-1
stimulation.
• Figure S2 SSH1L(W458A) retains the basal cofilin phosphatase activity but is poorly activated by F-actin.
• Figure S3 Effects of chick sr-LIMK1 (chLIMK) or S3E-cofilin expression on the phenotypes of LIMK1 siRNA cells.
• Video 1 (872 k) The three-dimensional projection of a Jurkat cell.
• Video 2 (3.5 MB) Time-lapse fluorescence microscopy of live Jurkat cells cotransfected with CFP-LIMK1 and YFP-SSH1L.
• Video 3 (2.4 MB) Time-lapse fluorescence microscopy of a live Jurkat cell cotransfected with YFP-actin and CFP-SSH1L.
• Video 4 (5.4 MB) Time-lapse fluorescence microscopy of a control Jurkat cell transfected with YFP-actin.
• Video 5 (5.4 MB) Time-lapse fluorescence microscopy of a cofilin siRNA Jurkat cell transfected with YFP-actin.
• Video 6 (5.4 MB) Time-lapse fluorescence microscopy of a LIMK1 siRNA Jurkat cell transfected with YFP-actin.
• Video 7 (5.1 MB) Time-lapse fluorescence microscopy of a SSH1L siRNA Jurkat cell transfected with YFP-actin.
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