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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200505107
Goshima et al.

Figure S1 Quantitation of K fiber focusing upon C-MTs depolymerization.

Figure S2 Abnormal spindle morphology upon double Ncd/Dhc64C RNAi is not a consequence of polyploidy.

Table S1

Video 1 GFP-tubulin dynamics in the metaphase spindle of an untreated ("wild-type") S2 cell.

Video 2 Another example of GFP-tubulin dynamics in a wild-type S2 cell.

Video 3 GFP-tubulin dynamics upon C-MTs depletion treatment in a wild-type S2 cell.

Video 4 GFP-tubulin dynamics upon colchicine treatment in a wild-type S2 cell.

Video 5 Ncd-GFP dynamics in the S2 metaphase spindle.

Video 6 Ncd-GFP-NES dynamics in interphase of a S2 cell.

Video 7 Ncd-GFP-NES dynamics in interphase after EB1 RNAi (day 7) in a S2 cell.

Video 8 Ncd-GFP dynamics in the S2 metaphase spindle after EB1 RNAi (day 5).

Video 9 Two image sequences produced by simulation.

Video 10 Dynein (green) supplemented with Ncd-EB1 (red-yellow), following the model in which the motor domain of Ncd binds to C-MTs.





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