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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200506124
Audhya et al.
• Figure S1 Both the CAR-1 Sm domain and RGG box bind to RNA-coated beads.
• Figure S2 CAR-1 and CGH-1 both localize to P-granules and smaller cytoplasmic particles.
• Figure S3 Microtubule density is reduced in the region between the centrosome and the chromosomes in metaphase spindles from CAR-1-depleted embryos.
• Figure S4 The absence of inter-zonal microtubules following car-1(RNAi) results in a defect in ZEN-4 localization.
• Table S1
• Video 1 The first mitotic division of a wild-type Caenorhabditis elegans embryo filmed using differential interference contrast (DIC) microscopy.
• Video 2 The first mitotic division of an embryo laid by a car-1(RNAi) hermaphrodite (RNA #194) filmed by DIC microscopy.
• Video 3 The first mitotic division of an embryo laid by a homozygous car-1(tm1753) hermaphrodite filmed by DIC microscopy.
• Video 4 The first 7 mitotic divisions of an embryo expressing GFPLAP:CAR-1 filmed by spinning disk confocal microscopy.
• Video 5 The first mitotic division of a wild-type (left) and a car-1(RNAi) embryo (dsRNA #194) (right) expressing GFP:PHPLC1
1, filmed by spinning disc confocal microscopy.
• Video 6 The first mitotic division of a wild-type embryo expressing both GFP:
-tubulin and GFP:PHPLC1
• Video 7 The first mitotic division of a wild-type (left) and a car-1(RNAi) embryo (RNA #194; right) expressing GFP:
• Video 8 The first mitotic division of a wild-type (left) and a car-1(RNAi) embryo (RNA# 194) (right) expressing GFP:
-tubulin and GFP:histone H2B filmed by spinning disk confocal microscopy.
• Video 9 The first mitotic division of a partial cgh-1(RNAi) embryo (RNA #273) expressing GFPLAP:CAR-1. Embryo was mounted in the absence of compression and filmed by spinning disk confocal microcopy.
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