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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200507116
Paladino et al.
• Figure S1 Targeting of NTR-PLAP and GFP-GPI at different days of culture.
• Figure S2 Analysis of the removal of GFP-GPI from the cell surface by trypsin by confocal microscopy and Western blotting.
• Figure S3 Tannic acid impairs transferrin internalization but does not affect surface binding.
• Figure S4 Apical and basolateral proteins are segregated in the TGN and travel in separate post-TGN carriers in fully polarized filter-grown MDCK cells.
• Figure S5 Internalization of GFP-GPI from the basolateral surface.
• Video 1 Time-lapse imaging in fully polarized MDCK cells of GFP-GPI from the TGN to the plasma membrane in the absence of tannic acid.
• Video 2 Time-lapse imaging in fully polarized MDCK cells of GFP-GPI from the TGN to the plasma membrane in the presence of tannic acid.
• Video 3 Time-lapse imaging in fully polarized MDCK cells of GFP-GPI from the TGN to the plasma membrane in the absence of tannic acid.
• Video 4 Time-lapse imaging in fully polarized MDCK cells of GFP-GPI from the TGN to the plasma membrane in the presence of tannic acid.
• Video 5 Time-lapse imaging in fully polarized MDCK cells of GFP-GPI from the TGN to the plasma membrane in the absence of tannic acid.
• Video 6 Time-lapse imaging in fully polarized MDCK cells of GFP-GPI from the TGN to the plasma membrane in the presence of tannic acid.
• Video 7 In vivo trafficking of GFP-GPI from the TGN to the plasma membrane in fully polarized MDCK cells.
• Video 8 n vivo trafficking of GFP-GPI from the TGN to the plasma membrane in fully polarized MDCK cells.
• Video 9 Budding and dynamics of GFP-GPI containing post-Golgi carriers in fully polarized MDCK cells.
• Supplemental text (PDF)
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