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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200508165
Nydegger et al.

Figure S1 Overall distribution of the tetraspanins CD9, CD81, CD82, and CD63 if the cells were either stained for only one of the antigens or dual-labeled for two tetraspanins.

Figure S2 Surface TEMs containing two tetraspanins.

Figure S3 Accumulation of viral Gag at plasma membrane TEMs containing CD9, CD81, or CD82.

Figure S4 TSG101 and VPS28, rather than accumulating at surface TEMs, are concentrated at intracellular compartments throughout the cell in the absence of viral Gag expression.

Figure S5 Immunofluorescence analysis of viral Env and Gag colocalization with tetraspanins.

Video 1 Untransfected HeLa cells that were surface stained for CD63.





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