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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200510032
Molk et al.

Figure S1 Kar3p-GFP localization during nuclear congression.

Figure S2 Cytoplasmic MT behavior in bilateral bik1{Delta} crosses.

Figure S3 Nuclear congression failure in kar3{Delta} cells labeled with GFP-Tub1p and Bik1p-3xGFP.

Video 1 A wild-type cell displayed persistent attachment of MTs to the shmoo tip, whereas a bik1{Delta} cell detached upon depolymerization.

Video 2 Bik1p-3xGFP expression in wild-type cells treated with mating pheromone.

Video 3 Mating between Bik1p-3xGFP-expressing cells.

Video 4 Wild-type cells expressing GFP-Tub1p were mated and imaged before nuclear congression.

Video 5 Bilateral kar9{Delta} cells expressing GFP-Tub1p were mated.

Video 6 Bik1p-3xGFP labeling was reduced on shmoo tip MTs in kip2{Delta} cells.

Video 7 Bik1p-3xGFP-labeled growing and shortening MT plus ends in kar3{Delta} cells.

Video 8 Nuclear congression in bik1{Delta} cells mated together when MTs were able to contact each other.

Video 9 kar3{Delta} cell expressing GFP-Tub1p mated to a kar3{Delta} cell with no integrated GFP tag.

Video 10 kar3-1 cell expressing GFP-Tub1p mated to wild-type cells expressing Bik1p-3xGFP.

Tables S1 and S2 (PDF)





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