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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200511093
Hotulainen et al.
• Figure S1 Overexpression of a loss-of-function mDia1/DRF1 mutant induces abnormal dorsal stress fiber morphology and accumulation of α-actinin in these structures.
• Figure S2 Expression of Scar-WA in U2OS cells leads to a loss of lamellipodia and transverse arcs.
• Video 1 Actin dynamics in a U2OS cell visualized by GFP-actin.
• Video 2 Actin dynamics in U2OS cells visualized with GFP-actin.
• Video 3 Ventral stress fiber assembly visualized with YFP-actin (green) and and zyxin-CFP (red).
• Video 4 Dorsal stress fiber elongation visualized by zyxin-CFP (red) and α-actinin-YFP (green) in U2OS cell.
• Video 5 Dorsal stress fibers and arcs visualized by zyxin-CFP (red) and α-actinin-YFP (green) in a U2OS cell.
• Video 6 Dorsal stress fibers and arcs visualized by zyxin-CFP (red) and α-actinin-YFP (green) in an mDia1/DRF1 siRNA-transfected U2OS cell.
• Video 7 Dorsal stress fibers and arcs visualized by zyxin-CFP (red) and α-actinin-YFP (green) in mDia1/DRF1 siRNA-transfected U2OS cell.
• Video 8 α-actinin-YFP intensity is shown to demonstrate arc assembly from short α-actinin cross-linked actin filament bundles generated at the leading lamellipodium.
• Video 9 Dorsal stress fibers visualized by GFP-actin in a p34 siRNA-transfected U2OS cell.
• Video 10 Effect of blebbistatin treatment in a U2OS cell expressing GFP-actin.
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