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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200512103
Schneider et al.
• Figure S1 GFP-GW and GFP expression in S2 cells.
• Figure S2 Flag-AGO2 colocalized and associated with endogenous Drosophila GW in S2 cells.
• Figure S3 RNAi knockdown of gw mRNA phenocopies the gw1 mutation.
• Video 1 Chromatin organization (labeled by histone-GFP) during early development
• Video 2 Abnormal pattern of chromosome division in a homozygous gw1 mutant expressing histone-GFP.
• Video 3 Histone-GFP-expressing embryos after the localized depletion of GW function by antibody injection
• Video 4 A tubulin-GFP fusion protein marks the localization of the spindles during early development in living Drosophila embryos.
• Video 5 Anti-GW antibody injection into the posterior pole of tubulin-GFP-expressing embryos at NC10.
• Video 6 The dynamic pattern of actin localization was monitored using the actin-binding domain of moesin-GFP expressed in live embryos.
• Video 7 Blocking GW function by anti-GW antibody injection at NC10 into moesin-GFP-expressing embryos leads to stabilization and then breakdown of the cortical actin network.
• Video 8 Injection of anti-GW antibody into embryos expressing NLS-GFP at NC10 causes progressive nuclear enlargement of the posterior pole and regional nuclear enlargement with each subsequent nuclear cycle.
• Video 9 Visualization of Drosophila GWBs in living S2 cells.
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