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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200602032
Wu et al.
• Figure S1 Unconventional, but not conventional, myosin II Myo2p colocalizes with the myosin II regulatory light chain Rlc1p spot.
• Figure S2 Profilin Cdc3p is required for the assembly of the contractile ring, but not of formin nodes.
• Figure S3 The absence of the anillin-like protein Mid1p and actin filaments favors the formation of aggregates of formin Cdc12p
• Video 1 The contractile ring forms from a broad band of nodes around the equator of the cell.
• Video 2 Rlc1p spots are not essential for the assembly of contractile ring.
• Video 3 Formin Cdc12p localizes to a broad band around the equator of the cell.
• Video 4 Contractile rings assemble from a broad band of formin Cdc12p nodes in synchronized cdc12-3GFP cdc25-22 cells (strain JW1118).
• Video 5 The formin Cdc12p accumulates in a broad band of nodes in the absence of actin filaments.
• Video 6 Profilin Cdc3p is required for the assembly of the contractile ring, but not formin nodes.
• Video 7 The PCH protein Cdc15p forms an equatorial broad band of nodes before coalescing laterally into a compact ring in strain JW1038 expressing GFP-Cdc15p.
• Video 8 YFP-actin concentrates in actin patches at the ends of cells until early in anaphase when the patches shift to the cell division site.
• Video 9 The Arp2/3 complex activator myosin I Myo1p concentrates in dynamic actin patches located at the cell tips and cell division site, but is not concentrated in the contractile ring.
• Video 10 Time course and dynamics of patch assembly.
• Table S1 S. pombe strains used in this study.
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