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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200604176
Miyoshi et al.
• Figure S1 Fast dissociation of Eps8 and VASP in lamellipodia.
• Figure S2 All Arp2/3 probes display identical localization in cells.
• Figure S3 Actin filament turnover is diminished by the overexpression of LIMK in XTC cells.
• Figure S4 Abundant barbed ends and pointed ends in lamellipodia.
• Figure S5 The speed of processive movement of the FH2 mutant of mDia1 in the cell peripheral area.
• Video 1 Time-lapse video of single-molecule speckles of CP (EGFP-CPβ1) at intervals of 500 ms.
• Video 2 Time-lapse video of single-molecule speckles of CP (EGFP-CPβ1) at intervals of 3 s.
• Video 3 Time-lapse video of full-length GFP-Eps8 expressed at a high level, showing its association with actin structures in lamellipodia and filopodia of an XTC cell.
• Video 4 Time-lapse video of single-molecule speckles of full-length GFP-Eps8 in lamellipodia acquired at intervals of 500 ms.
• Video 5 Time-lapse video of EGFP-VASP expressed at a high level, showing its association with the protruding leading edge of an XTC cell.
• Video 6 Time-lapse video of single-molecule speckles of EGFP-VASP in lamellipodia acquired at intervals of 300 ms.
• Video 7 Time-lapse video of single-molecule speckles of EGFP-gelsolin in lamellipodia acquired at intervals of 500 ms.
• Video 8 Time-lapse video of single-molecule speckles of the Arp2/3 complex (EGFP-p40) in lamellipodia of an XTC Xenopus fibroblast acquired at intervals of 3 s.
• Video 9 Rapid inhibition of the fast dissociation of CP speckles upon 1 µM Jas treatment.
• Video 10 Time-lapse video of EGFP-actin in an XTC cell expressing a high level of mRFP1-hLIMK-1.
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