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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200605006
Seth et al.

Figure S1 Membrane localization of the FRLα N terminus is inhibited by DAD binding in trans.

Figure S2 Localization of endogenous FRLα and expression of myc-tagged Rho GTPase mutants in RAW cells.

Figure S3 Characterization of cells treated with FRLα siRNA.

Figure S4 Additional images of FRLα recruitment to the phagocytic cup.

Table S1 Mean GFPm/GFPc ratios for all constructs and conditions tested

Video 1 DIC time-lapse video of an FRLα-GFP-expressing macrophage undergoing Fc-γ receptor-mediated phagocytosis.

Video 2 Fluorescence time-lapse video of an FRLα-GFP-expressing macrophage undergoing Fc-γ receptor-mediated phagocytosis.

Video 3 Magnified phagocytic cup

Video 4 DIC time-lapse video of a GBD-GFP-expressing macrophage undergoing Fc-γ receptor-mediated phagocytosis.

Video 5 Fluorescence time-lapse video of a GBD-GFP-expressing macrophage undergoing Fc-γ receptor-mediated phagocytosis.

 





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