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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200610005
Kiuchi et al.
• Figure S1 Expression of Dp-actin has no detrimental effect on actin organization in COS7 cells.
• Figure S2 LIMK1 expression markedly increases the level of cofilin phosphorylation in COS7 cells.
• Figure S3 Characterization of cofilin mutants.
• Figure S4 Microinjected actin monomers are less efficiently incorporated at the cell periphery without cell stimulation.
• Video 1 Time-lapse fluorescence of Dp in the COS7 cell shown in Fig. 1 A.
• Video 2 Time-lapse fluorescence of Dp-actin in the COS7 cells shown in Fig. 1, B and C, and Fig. 2, A and B.
• Video 3 Time-lapse fluorescence of Dp-actin in the COS7 cells coexpressing LIMK1 with cofilin mutants or Slingshot.
• Video 4 Time-lapse fluorescence of Dp-actin in the MCF-7 cells shown in Fig. 3 B.
• Video 5 Time-lapse fluorescence of Dp-actin in the cytoplasmic region in the RacV12-expressing N1E-115 cells, shown in Fig. 5.
• Video 6 Time-lapse fluorescence of Dp-actin photoactivated in the front region of the lamellipodium in the RacV12-expressing N1E-115 cell, shown in Fig. 6 A.
• Video 7 Time-lapse fluorescence of YFP-actin (left, green) and Alexa546-actin (middle, red) in the COS7 cells coexpressing YFP-actin and LIMK1(WT).
• Video 8 Time-lapse fluorescence of YFP-actin (left, green) and Alexa546-actin (middle, red) in the COS7 cells coexpressing YFP-actin and LIMK1(D460A).
• Video 9 Time-lapse fluorescence of YFP-actin (left, green) and Alexa546-actin (middle, red) in the MCF-7 cells coexpressing YFP-actin and cofilin/ADF siRNAs.
• Video 10 Time-lapse fluorescence of YFP-actin (left, green) and Alexa546-actin (middle, red) in the MCF-7 cells coexpressing YFP-actin and control siRNA.
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