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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200611046
Keizer-Gunnink et al.

Figure S1 Cell trajectories of wild-type cells in a gradient of cAMP (Video 1) or 8CPT-cAMP (Video 2).

Figure S2 Inhibition of PLC signaling by the antagonist 3′NH-cAMP.

Figure S3 Expression of PHcracGFP, PTEN-GFP, and PI3K-GFP in PLC-null cells in a gradient of cAMP or 8CPT-cAMP.

Video 1 Cell movement in a gradient of cAMP.

Video 2 Cell movement in a gradient of 8CPT-cAMP.

Video 3 Cell movement in gradients of cAMP and 8CPT-cAMP

Video 4 Cell movement and localization of actin and myosin in a gradient of cAMP

Video 5 Cell movement and localization of actin and myosin in a gradient of 8CPT-cAMP

Video 6 Upgradient localization of PHcracGFP (detecting PI[3,4,5]P3) and chemotaxis toward cAMP

Video 7 Downgradient localization of PHcracGFP and chemotaxis away from 8CPT cAMP

Video 8 Chemotaxis of pi3k1/2-null cells toward cAMP in the presence of 8CPT-cAMP

Video 9 Chemotaxis of PLC-null cells toward cAMP in the presence of 8CPT-cAMP





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