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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200611096
Xu et al.
• Figure S1 A previous gradient caused a temporary inverted PIP3 response in cells when they are exposed to a uniformly applied cAMP filed.
• Figure S2 Single-cell FRET measurement of heterotribmeric G protein dissociation, association, and re-dissociation in responses to cAMP.
• Figure S3 Reapplied a cAMP gradient induced an inverted PHCrac-GFP membrane translocation.
• Figure S4 Gα9 null cells detect cAMP gradient normally.
• Figure S5 Simulation of a cell that is exposed to a stable gradient at 0 s and gradually leads to a stronger inhibition of PI3K activity in the front than in the back while the cell develops PIP3 polarity.
• Video 1 Uniformly applied cAMP stimulation triggered inverted PHCrac-GFP translocation.
• Video 2 Uniformly applied cAMP stimulation triggered inverted PHCrac-GFP translocation.
• Video 3 Simultaneously measuring G protein activation in the front and back of a cell and the inverted PHCrac-GFP response.
• Video 4 Spatiotemporal dynamics of PTEN in single cells upon a withdrawal of a cAMP gradient and then reapplied the gradient.
• Video 5 A cAMP gradient (red) induced the inverted PHCrac-GFP (green) membrane translocation.
• Video 6 A cAMP gradient (red) induced the inverted PHCrac-GFP (green) membrane translocation.
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