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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200611138
Sasaki et al.

Figure S1 Ras and PI3K spontaneous activation in low density suspended cells.

Figure S2 Model for the Ras/PI3K circuit during random movement and chemotaxis.

Video 1 GFP-N-PI3K1 localization in null cells without extracellular stimuli.

Video 2 GFP-RBD localization in a null cell corresponds to Fig. 2 A in the text.

Video 3 GFP-RBD localization in a null cell corresponds to Fig. 2 D in the text.

Video 4 Simultaneous imaging of GFP-RBD and RFP-PHcrac in the wild-type vegetative cells corresponds to Fig. 3 A in the text.

Video 5 Random movement analysis of nonaxenic NC4 cells corresponds to Fig. 4 A in the text.

Video 6 Random movement analysis of cells corresponds to Fig. 4 A in the text.

Video 7 Random movement analysis of myristoylated PI3K2 (Myr.-PI3K2) overexpressing wild-type cells.

Video 8 GFP-RBD and RFP-PHcrac in the wild-type cells correspond to Fig. 4 B in the text.

Video 9 Random movement analysis of pianissimo null cells.

Video 10 GFP-RBD and RFP-PHcrac in the akt null cells.





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