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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200612043
Vicente-Manzanares et al.
• Figure S1 Spatial localization of myosin IIA and IIB in a migrating cell.
• Figure S2 Migratory phenotypes of MIIA-deficient (left column), MIIB-deficient (middle column), and control (right column) Rat2 fibroblasts.
• Figure S3 MIIA expression levels affect the number of adhesions.
• Video 1 Time-lapse movie of MIIA-deficient CHO.K1 cells.
• Video 2 Time-lapse movie of MIIB-deficient CHO.K1 cells.
• Video 3 Time-lapse movie of pSUPER-transfected (control) CHO.K1 cells.
• Video 4 Time-lapse movie of a MIIB-deficient CHO.K1 cell, highlighting nuclear spinning.
• Video 5 Dual-color TIRF time-lapse movie of a protrusion of a MIIB-deficient CHO.K1 cell cotransfected with mChe-MIIB (magenta) and paxillin-GFP (green).
• Video 6 TIRF time-lapse movie of a protrusion of a pSUPER-transfected control CHO.K1 cell cotransfected with paxillin-GFP.
• Video 7 TIRF time-lapse movie of a protrusion of a MIIA-deficient CHO.K1 cell cotransfected with paxillin-GFP.
• Video 8 TIRF time-lapse movie of a protrusion of a MIIB-deficient CHO.K1 cell cotransfected with paxillin-GFP.
• Video 9 TIRF time-lapse movie of a protrusion of a MIIA-deficient CHO.K1 cell cotransfected with paxillin-GFP.
• Video 10 TIRF time-lapse movie of a MIIA-deficient CHO.K1 cell cotransfected with paxillin-GFP (shown).
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