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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200702062
Griffis et al.
• Figure S1 The endogenous Spindly protein also enriches on unattached, unaligned, and anaphase kinetochores
• Figure S2 Kymograph analysis of GFP-Spindly particles.
• Figure S3 Spindly depletion does not alter the targeting of endogenous dynactin to the kinetochore.
• Figure S4 Spindly is not required for the dynein-dependent reorganization of endosomes in S2 cells.
• Figure S5 The depletion of NP_060255 causes defects in chromosome alignment.
• Video 1 GFP-Spindly tracks on the plus ends of microtubules in interphase cells.
• Video 2 GFP-Spindly concentrates on lagging chromosomes and diminishes after alignment at the metaphase plate.
• Video 3 GFP Spindly returns to kinetochores during anaphase.
• Video 4 GFP-Spindly traffics from kinetochores to centrosomes in a dynein- and Rod-dependent manner.
• Video 5 The depletion of Spindly, dynein, or Rod slows the alignment of chromosomes on the metaphase plate.
• Video 6 The depletion of Spindly, dynein, or Rod slows the alignment of chromosomes on the metaphase plate.
• Video 7 The depletion of Spindly, dynein, or Rod slows the alignment of chromosomes on the metaphase plate.
• Video 8 The depletion of Spindly, dynein, or Rod slows the alignment of chromosomes on the metaphase plate.
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