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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200705068
Jeon et al.

Figure S1 Sequence and expression kinetics of RapGAP1 and Ras activation kinetics in rapGAP1 and RapGAP1OE cells.

Figure S2 GFP-RapGAP1–overexpressing cells show more frequent changes in direction.

Figure S3 GFP-RapGAP1–overexpressing cells show more frequent changes in direction.

Video 1 Chemotaxis of wild-type (KAx-3) cells toward a micropipette emitting the chemoattractant cAMP.

Video 2 Chemotaxis of rapGAP1 cells to a micropipette emitting the chemoattractant cAM

Video 3 Chemotaxis of rapGAP1 cells expressing GFP-RapGAP1 to a micropipette emitting the chemoattractant cAMP.

Video 4 Spatial localization of RalGDS-YFP and RFP-RapGAP1 in chemotaxing cells.

Video 5 Spatial localization of RalGDS-YFP and RFP-coronin in chemotaxing cells.

Video 6 Actin foci at the bottom of the cells expressing GFP-RapGAP1 as observed by confocal microscopy.

Video 7 Chemotaxis of ctxA/B cells to a micropipette emitting the chemoattractant cAMP.





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