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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200707009
Sidani et al.
• Figure S1 Analysis of the cofilin siRNA shows high efficiency in suppressing cofilin levels with specificity.
• Figure S2 The directionality and persistence of cofilin knockdown cells can be rescued by re-expression of human cofilin plasmid.
• Figure S3 Perturbing cofilin levels by overexpression of LIMK in MTLn3 changes their motility behavior.
• Figure S4 Cofilin knockdown suppresses EGF-induced protrusion, but does not affect the presence of the lamellipodium.
• Figure S5 The elongated phenotype of cofilin knockdown cells could not be explained by myosin II accumulation at the rear end of the cells.
• Video 1 Control MTLn3 cells exhibit random walking behavior.
• Video 2 Cofilin siRNA knockdown cells show a directional motility behavior.
• Video 3 Expression of the WT(ΔH) cofilin plasmid rescues the directional walking behavior characteristic of the cofilin siRNA knockdown cells.
• Video 4 Upon EGF stimulation, control MTLn3 cells exhibit lamellipod extension and accumulation of F-actin at the leading edge of the lamellipod.
• Video 5 Cofilin siRNA knockdown suppresses EGF-induced F-actin assembly and protrusion in MTLn3 cells.
• Video 6 Expression of the WT(ΔH) cofilin plasmid rescues the suppression of the EGF-induced F-actin assembly and protrusion observed in the cofilin knockdown cells.
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