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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200709029
Bartolini et al.
• Figure S1 FH1FH2mDia2 stabilizes MTs independently of its tag.
• Figure S2 His-FH1FH2mDia2 bundles MTs in vitro.
• Figure S3 Actin assembly kinetics using the indicated concentrations of His-tagged wild type (FH1FH2) and the deletion fragment FH2C of mDia2.
• Video 1 Time lapse of EGFP-FH1FH2mDia2 dynamics in NIH3T3 by TIRF microscopy.
• Video 2 Time lapse of W853A EGFP-FH1FH2mDia2 dynamics in NIH3T3 by TIRF microscopy.
• Video 3 Time lapse of I704A EGFP-FH1FH2mDia2 dynamics in NIH3T3 by TIRF microscopy.
• Video 4 Time lapse of W630A EGFP-FH1FH2mDia2 dynamics in NIH3T3 by TIRF microscopy.
• Video 5 Real-time DIC microscopy video recording of axoneme-nucleated MTs undergoing dilution-induced disassembly in the presence of FH1FH2mDia2.
• Video 6 Real-time DIC microscopy video recording of axoneme-nucleated MTs undergoing dilution-induced disassembly in the presence of HKCL buffer.
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