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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200709134
Nemethova et al.
• Figure S1 Electron micrographs of negatively stained filopodia in CAR fish fibroblasts.
• Figure S2 Electron micrograph
• Figure S3 Electron micrograph
• Video 1 Actin dynamics in a CAR fibroblast transfected with mCherry-actin.
• Video 2 Actin dynamics in a CAR fibroblast transfected with mCherry-actin.
• Video 3 Transition of filopodia into the lamella visualized in a CAR fibroblast transfected with mCherry-actin and EGFP-fascin.
• Video 4 Entry of filopodia into ventral layer of the cytoskeleton.
• Video 5 Correlative live cell imaging for electron microscopy, including the image after fixation.
• Video 6 Myosin contractility is required for the integration of a filopodium into the lamella cytoskeleton.
• Video 7 CAR fibroblast before and after treatment with 50 µM blebbistatin.
• Video 8 CAR fibroblast before and after treatment with 30 µM of Rho-kinase inhibitor.
• Video 9 Filopodia are involved in the initiation of substrate adhesion and stress fibers.
• Video 10 B16 melanoma cell before and after treatment with 30 µM blebbistatin.
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