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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200710111
Mondal et al. Figure S1 Scheme for generation of gefQ− cells and verification of mutants by genomic PCR, Southern blotting, Western blotting, and immunofluorescence studies.
Figure S2 RasB activation in gefQ− cells expressing the GFP-Δ-GEF-GEFQ.
Video 1 Live cell imaging of AX2 cells overexpressing GFP-RasGEF Q173.
Video 2 Live cell imaging of AX2 cells overexpressing GFP-RasGEF Q173.
Video 3 Live cell imaging of AX2 cells overexpressing GFP-RasGEF Q173.
Video 4 Live cell imaging of AX2 cells overexpressing GFP-RasGEF Q173.
Video 5 Live cell imaging of AX2 cells overexpressing GFP-GEF.
Video 6 Live cell imaging of AX2 cells overexpressing GFP-DEP.
Video 7 Live cell imaging of wild-type AX2 cells during development on a phosphate agar plate.
Video 8 Live cell imaging of gefQ− cells during development on a phosphate agar plate.
Video 9 Migration of aggregation-competent wild-type AX2 cells toward a cAMP source.
Video 10 Migration of aggregation-competent gefQ− cells toward a cAMP source.
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