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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200710195
Mahadevaiah et al. • Figure S1 Immunostaining for the DSB-associated proteins RPA and RAD51, both of which are known to localize to the single-stranded DNA tails generated by resection of the SPO11-generated meiotic DSBs.
• Figure S2 Assessment of Atr, Brca1, Zfy2, and Ddx3x/Usp9x transcription in spermatocytes by RNA FISH, with expression in spermatids serving as a positive control.
• Figure S3 Zfy2 and Ddx3x/Usp9x transcription in Brca1Δ11/Δ11 pachytene spermatocytes.
• Figure S4 RNA FISH evidence for MSCI failure in Brca1 mutant testes and evidence of DSB repair after self-synapsis of the h21 chromosome.
• Figure S5 The onset of H1t expression at testis epithelial stage IV and the elimination of pachytene spermatocytes at this stage in H2ax- and Dnmt3l-null mice.
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