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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200712066
author et al.
• Figure S1 Validation that Gαi3 is preferentially localized within pseudopods at the leading edge during cell migration using DiD as a PM marker.
• Figure S2 Depletion of Gαi3 impairs EGF-stimulated Akt activation.
• Figure S3 Insulin-stimulated actin remodeling is inhibited in Gαi3-depleted cells.
• Figure S4 GIV is occasionally distributed along stress fibers in epithelial cells.
• Figure S5 The activation state of the G protein, not the phosphorylation of GIV at S1416, regulates the Gαi3–GIV interaction.
• Video 1 HeLa cells expressing rGαi3-YFP demonstrate directional migration toward the wound, whereas Gαi3-depleted cells show random motility.
• Video 2 Gαi3-YFP preferentially localizes within pseudopods at the leading edge of a migrating HeLa cell.
• Video 3 Mp-YFP is distributed along the plasma membrane during polarized cell migration, with no preference for the leading edge.
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