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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200712138
Shimada et al.
• Figure S1 L1 is linked to actin filament retrograde flow in axonal growth cones.
• Figure S2 Effects of shootin1 RNAi and NES-ΔN-shootin1 overexpression on actin filament retrograde flow in axonal growth cones.
• Figure S3 Effects of RNAi-refractory shootin1 and LY294002 on the shootin1-RNAi–induced inhibition of axon outgrowth on L1-Fc.
• Figure S4 Effects of shootin1 overexpression on actin filament retrograde flow and of LY294002 on shootin1-induced promotion of L1-dependent neurite outgrowth.
• Figure S5 Repression of L1 by RNAi.
• Video 1 Time-lapse fluorescent speckle images of EGFP-shootin1 in an axonal growth cone.
• Video 2 Time-lapse fluorescent speckle images of EGFP-shootin1 expressed in an XTC fibroblast.
• Video 3 Time-lapse fluorescent speckle images of mCherry-β-actin (red) and EGFP-shootin1 (green) coexpressed in an XTC fibroblast.
• Video 4 Time-lapse fluorescent speckle images of mCherry-β-actin (red) and EGFP-shootin1 (green) coexpressed in XTC fibroblasts treated with 1 µM cytochalasin D.
• Video 5 Time-lapse fluorescent speckle images of EGFP-shootin1 in an axonal growth cone treated with 1 μM cytochalasin D.
• Video 6 Time-lapse DIC images showing retrograde movement of L1-Fc–coated beads on an axonal growth cone (two days in vitro) expressing a control shRNA.
• Video 7 Time-lapse DIC images showing retrograde movement of L1-Fc–coated beads on an axonal growth cone (two days in vitro) expressing the shRNA designated against shootin1.
• Video 8 Time-lapse fluorescent speckle images of EGFP-ΔN-shootin1 expressed in a XTC fibroblast.
• Video 9 Time-lapse fluorescent speckle images of EGFP-shootin1 and mCherry-actin coexpressed in the same XTC fibroblast.
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