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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200801027
Kueh et al.
• Figure S1 Analysis of polymer mass decay in filament bundles grown off L. polyphemus acrosomal processes.
• Figure S2 Rapid monomer-insensitive disassembly of single actin filaments requires cofilin and coronin in addition to Aip1.
• Figure S3 CytoD likely does not inhibit filament disassembly by acting in solution.
• Figure S4 Diagram of mRFP-PAGFP-actin construct used for photoactivation experiments in cells.
• Video 1 Streaming video of the disassembly of single actin filaments in the presence of 2 µM cofilin, 1 µM coronin, 200 nM Aip1, 5 µM actin, and 2 mM ATP.
• Video 2 Time-lapse video of disassembly of an filament bundle grown off L. polyphemus acrosomal processes.
• Video 3 Time-lapse video of moving L. monocytogenes in GFP-actin BSC-1 cells treated with CytoD.
• Video 4 Time-lapse video of moving L. monocytogenes in GFP-actin BSC-1 cells treated with KabC.
• Video 5 Time-lapse video of moving L. monocytogenes in GFP-actin BSC-1 cells treated with LatB.
• Video 6 Time-lapse video of ruffling BSC-1 cells expressing PAGFP-mRFP-actin.
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