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Index of Online Supplemental Material for
J. Cell Biol. 10.1083/jcb.200801049
Osmundson et al.
• Figure S1 Centrosomal localization of endogenous and transfected Smurf2 examined by multiple antibodies.
• Figure S2 Multinucleation and misaligned chromosomes in HeLa cells with Smurf2 depletion.
• Figure S3 Down-regulation of endogenous and transfected Mad2 in cells with Smurf2 depletion.
• Figure S4 Control immunoblots for Fig. 5 c.
• Figure S5 Overexpression of wild-type Smurf2 minimally affects levels of securin, cyclin B1, and Mad2.
• Video 1 Impaired mitotic progression, failed cytokinesis, and binucleation in Smurf2 siRNA (siSmurf2)–transfected HeLa cells demonstrated by time-lapse phase microscopy.
• Video 2 Defective chromosomal alignment in Smurf2-depleted (siSmurf2) GFP-H2B HeLa cells at metaphase demonstrated by time-lapse fluorescence microscopy.
• Video 3 Premature anaphase onset in Smurf2-depleted (siSmurf2) GFP-H2B HeLa cells demonstrated by time-lapse fluorescence microscopy.
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